Please use this identifier to cite or link to this item: https://doi.org/10.1167/iovs.11-8260
Title: Subretinal Delivery of Ultrathin Rigid-Elastic Cell Carriers Using a Metallic Shooter Instrument and Biodegradable Hydrogel Encapsulation
Authors: Stanzel, Boris V
LIU ZENGPING 
Brinken, Ralf
Braun, Norbert
Holz, Frank G
Eter, Nicole
Keywords: Science & Technology
Life Sciences & Biomedicine
Ophthalmology
RETINAL-PIGMENT EPITHELIUM
ANTERIOR LENS CAPSULE
HUMAN BRUCHS MEMBRANE
MACULAR DEGENERATION
PHARMACOLOGICAL VITREOLYSIS
FLUID RESORPTION
PROGENITOR CELLS
TRANSPLANTATION
TISSUE
SPACE
Issue Date: 1-Jan-2012
Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Citation: Stanzel, Boris V, LIU ZENGPING, Brinken, Ralf, Braun, Norbert, Holz, Frank G, Eter, Nicole (2012-01-01). Subretinal Delivery of Ultrathin Rigid-Elastic Cell Carriers Using a Metallic Shooter Instrument and Biodegradable Hydrogel Encapsulation. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 53 (1) : 490-500. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.11-8260
Abstract: PURPOSE: To develop a surgical technique for the subretinal implantation of cell carriers suitable for the transplantation of cultured retinal pigment epithelium (RPE) in a preclinical animal model. METHODS: Cell carriers were porous 10-μm-thick polyester membranes. A custom-made shooter instrument consisted of a 20-gauge metallic nozzle with a nonstick plunger. Fetal human RPE cultures were used for vitality assessment during instrument handling. Transvitreal subretinal implantation of carriers without RPE was performed in 31 rabbits after vitrectomy. Fourteen of 31 implants were encapsulated in gelatin. Fluid turbulence over the implantation site was minimized using a novel infusion cannula. Six rabbits had intravitreal plasmin injections before surgery. SD-OCT in vivo images were obtained after 3, 7, and 14 days, followed by perfusion-fixed histology. RESULTS: Gelatin encapsulation of RPE/polyester implants made cell loss during handling reproducible, compared with 40% of controls showing random, large damage zones. Gelatin implants were ejected smoothly in 12 of 14 surgeries (86%), whereas "naked" implants frequently became trapped with the instrument, which reduced success to 9 of 17 cases (53%). Vitreous remnants after vitrectomy alone complicated subretinal placement of encapsulated and naked implants in 7 of 25 cases (28%). Plasmin-assisted vitrectomy resulted in implant ejection unperturbed by vitreous adhesions in six experiments. SD-OCT and histology demonstrated atraumatic subretinal implant delivery after uncomplicated surgery. CONCLUSIONS: A novel shooter instrument design allows for safe and atraumatic transvitreal delivery of hydrogel-encapsulated, ultrathin, rigid-elastic carriers into the subretinal space. The procedure may be used in the future to deliver cultured RPE.
Source Title: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
URI: https://scholarbank.nus.edu.sg/handle/10635/206745
ISSN: 0146-0404
1552-5783
DOI: 10.1167/iovs.11-8260
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