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https://doi.org/10.3791/53927
Title: | A Step by Step Protocol for Subretinal Surgery in Rabbits | Authors: | Al-Nawaiseh, Sami Thieltges, Fabian LIU ZENGPING Strack, Claudine Brinken, Ralf Braun, Norbert Wolschendorf, Marc Maminishkis, Arvydas Eter, Nicole Stanzel, Boris V |
Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics Medicine Issue 115 age-related macular degeneration cell carrier cell replacement SD-OCT pluripotent stem cells rabbit retinal pigment epithelium vitreoretinal surgery transplantation tissue engineering vitrectomy ARTIFICIAL RETINAL-DETACHMENT PIGMENT EPITHELIAL-CELLS BRUCHS MEMBRANE TRANSPLANTATION RPE SPACE SHEETS FLUID IMPLANTATION SCAFFOLDS |
Issue Date: | 1-Sep-2016 | Publisher: | JOURNAL OF VISUALIZED EXPERIMENTS | Citation: | Al-Nawaiseh, Sami, Thieltges, Fabian, LIU ZENGPING, Strack, Claudine, Brinken, Ralf, Braun, Norbert, Wolschendorf, Marc, Maminishkis, Arvydas, Eter, Nicole, Stanzel, Boris V (2016-09-01). A Step by Step Protocol for Subretinal Surgery in Rabbits. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2016 (115). ScholarBank@NUS Repository. https://doi.org/10.3791/53927 | Abstract: | Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision. Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology. The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models. | Source Title: | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | URI: | https://scholarbank.nus.edu.sg/handle/10635/206741 | ISSN: | 1940-087X | DOI: | 10.3791/53927 |
Appears in Collections: | Staff Publications Elements |
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