Please use this identifier to cite or link to this item: https://doi.org/10.3791/53927
Title: A Step by Step Protocol for Subretinal Surgery in Rabbits
Authors: Al-Nawaiseh, Sami
Thieltges, Fabian
LIU ZENGPING 
Strack, Claudine
Brinken, Ralf
Braun, Norbert
Wolschendorf, Marc
Maminishkis, Arvydas
Eter, Nicole
Stanzel, Boris V
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Medicine
Issue 115
age-related macular degeneration
cell carrier
cell replacement
SD-OCT
pluripotent stem cells
rabbit
retinal pigment epithelium
vitreoretinal surgery
transplantation
tissue engineering
vitrectomy
ARTIFICIAL RETINAL-DETACHMENT
PIGMENT EPITHELIAL-CELLS
BRUCHS MEMBRANE
TRANSPLANTATION
RPE
SPACE
SHEETS
FLUID
IMPLANTATION
SCAFFOLDS
Issue Date: 1-Sep-2016
Publisher: JOURNAL OF VISUALIZED EXPERIMENTS
Citation: Al-Nawaiseh, Sami, Thieltges, Fabian, LIU ZENGPING, Strack, Claudine, Brinken, Ralf, Braun, Norbert, Wolschendorf, Marc, Maminishkis, Arvydas, Eter, Nicole, Stanzel, Boris V (2016-09-01). A Step by Step Protocol for Subretinal Surgery in Rabbits. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2016 (115). ScholarBank@NUS Repository. https://doi.org/10.3791/53927
Abstract: Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision. Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology. The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models.
Source Title: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
URI: https://scholarbank.nus.edu.sg/handle/10635/206741
ISSN: 1940-087X
DOI: 10.3791/53927
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