Please use this identifier to cite or link to this item: https://doi.org/10.1021/acsomega.7b01604
Title: Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering
Authors: Chan, Siew Yin
Chan, Benjamin Qi Yu
LIU ZENGPING 
Parikh, Bhav Harshad
Zhang, Kangyi
Lin, Qianyu
SU XINYI 
Kai, Dan
Choo, Wee Sim
Young, David James
Loh X.J 
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
CRYSTALLIZATION BEHAVIOR
INFRARED-SPECTROSCOPY
STEM-CELL
ANTIOXIDANT
SCAFFOLDS
CHITOSAN
TRANSPLANTATION
CRYSTALLINITY
DEGRADATION
MISCIBILITY
Issue Date: 1-Dec-2017
Publisher: AMER CHEMICAL SOC
Citation: Chan, Siew Yin, Chan, Benjamin Qi Yu, LIU ZENGPING, Parikh, Bhav Harshad, Zhang, Kangyi, Lin, Qianyu, SU XINYI, Kai, Dan, Choo, Wee Sim, Young, David James, Loh X.J (2017-12-01). Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering. ACS OMEGA 2 (12) : 8959-8968. ScholarBank@NUS Repository. https://doi.org/10.1021/acsomega.7b01604
Abstract: Natural polysaccharide pectin has for the first time been grafted with polyhydroxybutyrate (PHB) via ring-opening polymerization of β-butyrolactone. This copolymer, pectin-polyhydroxybutyrate (pec-PHB), was blended with PHB in various proportions and electrospun to produce nanofibers that exhibited uniform and beadfree nanostructures, suggesting the miscibility of PHB and pec-PHB. These nanofiber blends exhibited reduced fiber diameters from 499 to 336'426 nm and water contact angles from 123.8 to 88.2 on incorporation of pec-PHB. They also displayed 39'335% enhancement of elongation at break relative to pristine PHB nanofibers. pec-PHB nanofibers were found to be noncytotoxic and biocompatible. Human retinal pigmented epithelium (ARPE-19) cells were seeded onto pristine PHB and pec-PHB nanofibers as scaffold and showed good proliferation. Higher proportions of pec-PHB (pec-PHB10 and pec-PHB20) yielded higher densities of cells with similar characteristics to normal RPE cells. We propose, therefore, that nanofibers of pec-PHB have significant potential as retinal tissue engineering scaffold materials.
Source Title: ACS OMEGA
URI: https://scholarbank.nus.edu.sg/handle/10635/206738
ISSN: 2470-1343
DOI: 10.1021/acsomega.7b01604
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