Please use this identifier to cite or link to this item: https://doi.org/10.1167/tvst.10.1.10
Title: Hints for Gentle Submacular Injection in Non-Human Primates Based on Intraoperative OCT Guidance
Authors: Tan, Gavin SW
LIU ZENGPING 
Ilmarinen, Tanja
VELUCHAMY AMUTHA BARATHI
Chee, Caroline K
LINGAM GOPAL
SU XINYI 
Stanzel, Boris V
Keywords: Science & Technology
Life Sciences & Biomedicine
Ophthalmology
submacular surgery
fovea
non-human primates
intraoperative OCT
Advanced Therapy Medicinal Products
Issue Date: 1-Jan-2021
Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Citation: Tan, Gavin SW, LIU ZENGPING, Ilmarinen, Tanja, VELUCHAMY AMUTHA BARATHI, Chee, Caroline K, LINGAM GOPAL, SU XINYI, Stanzel, Boris V (2021-01-01). Hints for Gentle Submacular Injection in Non-Human Primates Based on Intraoperative OCT Guidance. TRANSLATIONAL VISION SCIENCE & TECHNOLOGY 10 (1). ScholarBank@NUS Repository. https://doi.org/10.1167/tvst.10.1.10
Abstract: Purpose: Delivery of Advanced Therapy Medicinal Products to the submacular space is increasingly evolving into a therapeutic modality. Cell replacement for age-related macular degeneration (AMD) and gene therapy for RPE65 are recent successful examples. Herein, a nonhuman primate (NHP) model was used to investigate surgical means to detach the macula. Methods: Sixteen eyes of 13 healthy macaques underwent a 25-gauge vitrectomy and subretinal injection of balanced salt solution monitored by microscope-integrated intra-operative optical coherence tomography (miOCT). The animals were followed with OCT and histology. Results: The miOCT monitoring allowed a more precise definition of surgical trauma ranging from an initial full-thickness foveal tear, or induction of a cystoid macular edema (CME), until no foveal defect was discernible, as the technique improved. However, as the subretinal fluid wave detached the fovea, the aforementioned lesions formed, whereas persistent retinal adhesion reproducibly proved to remain in the distal parafoveal semi-annulus. Measures to reduce foveal trauma during submacular fluid injection included reducing intraocular pressure, injection volume, and velocity, as well as the retinal location for bleb initiation, use of a vitreous tamponade, and a dual-bore subretinal cannula. Conclusions: A stable very low intraocular pressure and careful subretinal injection may avoid tangential macular stretching or mechanical CME formation, while vitreous tamponade may facilitate a more lamellar subretinal flow, all thereby reducing foveal trauma during submacular injection in NHP. Translational Relevance: These results can be relevant to any submacular surgery procedure used today, as they synergistically reduce the risk of compromising foveal integrity.
Source Title: TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/206732
ISSN: 2164-2591
DOI: 10.1167/tvst.10.1.10
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Hints for Gentle Submacular Injection in Non-Human Primates Based on Intraoperative OCT Guidance.pdfPublished version7.99 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

5
checked on May 14, 2022

Page view(s)

52
checked on May 12, 2022

Download(s)

1
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.