Please use this identifier to cite or link to this item: https://doi.org/10.1084/jem.20180421
Title: Ca2+- mitochondria axis drives cell division in hematopoietic stem cells
Authors: Umemoto, Terumasa
Hashimoto, Michihiro
Matsumura, Takayoshi
Nakamura-Ishizu, Ayako
Suda, Toshio
Keywords: Science & Technology
Life Sciences & Biomedicine
Immunology
Medicine, Research & Experimental
Research & Experimental Medicine
SELF-RENEWAL
MAINTENANCE
QUIESCENCE
CALCIUM
RECEPTORS
ADENOSINE
NICHE
DIFFERENTIATION
PROLIFERATION
HOMEOSTASIS
Issue Date: 1-Aug-2018
Publisher: ROCKEFELLER UNIV PRESS
Citation: Umemoto, Terumasa, Hashimoto, Michihiro, Matsumura, Takayoshi, Nakamura-Ishizu, Ayako, Suda, Toshio (2018-08-01). Ca2+- mitochondria axis drives cell division in hematopoietic stem cells. JOURNAL OF EXPERIMENTAL MEDICINE 215 (8) : 2097-2113. ScholarBank@NUS Repository. https://doi.org/10.1084/jem.20180421
Abstract: Most of the hematopoietic stem cells (HSCs) within the bone marrow (BM) show quiescent state with a low mitochondrial membrane potential (ΔΨm). In contrast, upon stress hematopoiesis, HSCs actively start to divide. However, the underlying mechanism for the initiation of HSC division still remains unclear. To elucidate the mechanism underlying the transition of cell cycle state in HSCs, we analyzed the change of mitochondria in HSCs after BM suppression induced by 5-fluoruracil (5-FU). We found that HSCs initiate cell division after exhibiting enhanced ΔΨm as a result of increased intracellular Ca2+ level. Although further activation of Ca2+–mitochondria pathway led to loss of HSCs after cell division, the appropriate suppression of intracellular Ca2+ level by exogenous adenosine or Nifedipine, a Ca2+ channel blocker, prolonged cell division interval in HSCs, and simultaneously achieved both cell division and HSC maintenance. Collectively, our results indicate that the Ca2+–mitochondria pathway induces HSC division critically to determine HSC cell fate.
Source Title: JOURNAL OF EXPERIMENTAL MEDICINE
URI: https://scholarbank.nus.edu.sg/handle/10635/206157
ISBN: 15409538
ISSN: 00221007
DOI: 10.1084/jem.20180421
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