Please use this identifier to cite or link to this item: https://doi.org/10.3390/v12111239
Title: Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX
Authors: Liu, H. 
Zhou, Y.
Xu, Q.
Wong, Sek, Man 
Keywords: aptamers
emulsion PCR
RBSDV
rice black-streaked dwarf virus
SELEX
Issue Date: 2020
Publisher: NLM (Medline)
Citation: Liu, H., Zhou, Y., Xu, Q., Wong, Sek, Man (2020). Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX. Viruses 12 (11). ScholarBank@NUS Repository. https://doi.org/10.3390/v12111239
Rights: Attribution 4.0 International
Abstract: Rice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production. RBSDV is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallén) in a persistent manner. In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA. All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. This is the first report of aptamers being selected for detection of a rice virus capsid protein.
Source Title: Viruses
URI: https://scholarbank.nus.edu.sg/handle/10635/199549
ISSN: 1999-4915
DOI: 10.3390/v12111239
Rights: Attribution 4.0 International
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