Please use this identifier to cite or link to this item: https://doi.org/10.3390/v12111239
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dc.titleSelection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX
dc.contributor.authorLiu, H.
dc.contributor.authorZhou, Y.
dc.contributor.authorXu, Q.
dc.contributor.authorWong, Sek, Man
dc.date.accessioned2021-08-26T07:31:50Z
dc.date.available2021-08-26T07:31:50Z
dc.date.issued2020
dc.identifier.citationLiu, H., Zhou, Y., Xu, Q., Wong, Sek, Man (2020). Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX. Viruses 12 (11). ScholarBank@NUS Repository. https://doi.org/10.3390/v12111239
dc.identifier.issn1999-4915
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/199549
dc.description.abstractRice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production. RBSDV is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallén) in a persistent manner. In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA. All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. This is the first report of aptamers being selected for detection of a rice virus capsid protein.
dc.publisherNLM (Medline)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2020
dc.subjectaptamers
dc.subjectemulsion PCR
dc.subjectRBSDV
dc.subjectrice black-streaked dwarf virus
dc.subjectSELEX
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.3390/v12111239
dc.description.sourcetitleViruses
dc.description.volume12
dc.description.issue11
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