Please use this identifier to cite or link to this item: https://doi.org/10.3389/fimmu.2020.553362
Title: Humanized Mouse as a Tool to Predict Immunotoxicity of Human Biologics
Authors: Yong, K.S.M.
Her, Z.
Tan, S.Y.
Tan, W.W.S.
Liu, M.
Lai, F.
Heng, S.M.
Fan, Y.
Chang, K.T.E. 
Wang, C.-I.
Chan, J.K.Y. 
Chen, J.
Chen, Q. 
Keywords: biologic testing
cytokine storm
humanized mice
immunotoxicity
inflammation
Issue Date: 2020
Publisher: Frontiers Media S.A.
Citation: Yong, K.S.M., Her, Z., Tan, S.Y., Tan, W.W.S., Liu, M., Lai, F., Heng, S.M., Fan, Y., Chang, K.T.E., Wang, C.-I., Chan, J.K.Y., Chen, J., Chen, Q. (2020). Humanized Mouse as a Tool to Predict Immunotoxicity of Human Biologics. Frontiers in Immunology 11 : 553362. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2020.553362
Rights: Attribution 4.0 International
Abstract: Advancements in science enable researchers to constantly innovate and create novel biologics. However, the use of non-human animal models during the development of biologics impedes identification of precise in vivo interactions between the human immune system and treatments. Due to lack of this understanding, adverse effects are frequently observed in healthy volunteers and patients exposed to potential biologics during clinical trials. In this study, we evaluated and compared the effects of known immunotoxic biologics, Proleukin®/IL-2 and OKT3 in humanized mice (reconstituted with human fetal cells) to published clinical outcomes. We demonstrated that humanized mice were able to recapitulate in vivo pathological changes and human-specific immune responses, such as elevated cytokine levels and modulated lymphocytes and myeloid subsets. Given the high similarities of immunological side effects observed between humanized mice and clinical studies, this model could be used to assess immunotoxicity of biologics at a pre-clinical stage, without placing research participants and/or patients at risk. © Copyright © 2020 Yong, Her, Tan, Tan, Liu, Lai, Heng, Fan, Chang, Wang, Chan, Chen and Chen.
Source Title: Frontiers in Immunology
URI: https://scholarbank.nus.edu.sg/handle/10635/199445
ISSN: 16643224
DOI: 10.3389/fimmu.2020.553362
Rights: Attribution 4.0 International
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