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Title: Comparative Study of Curvature Sensing Mediated by F-BAR and an Intrinsically Disordered Region of FBP17
Authors: Su, M.
Zhuang, Y.
Miao, X.
Zeng, Y.
Gao, W.
Zhao, W.
Wu, M. 
Keywords: Biological Sciences
Cell Biology
Issue Date: 2020
Publisher: Elsevier Inc.
Citation: Su, M., Zhuang, Y., Miao, X., Zeng, Y., Gao, W., Zhao, W., Wu, M. (2020). Comparative Study of Curvature Sensing Mediated by F-BAR and an Intrinsically Disordered Region of FBP17. iScience 23 (11) : 101712. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Membrane curvature has emerged as an intriguing physical principle underlying biological signaling and membrane trafficking. The CIP4/FBP17/Toca-1 F-BAR subfamily is unique in the BAR family because its structurally folded F-BAR domain does not contain any hydrophobic motifs that insert into membrane. Although widely assumed so, whether the banana-shaped F-BAR domain alone can sense curvature has never been experimentally demonstrated. Using a nanobar-supported lipid bilayer system, we found that the F-BAR domain of FBP17 displayed minimal curvature sensing in vitro. In comparison, an alternatively spliced intrinsically disordered region (IDR) adjacent to the F-BAR domain has the membrane curvature-sensing ability greatly exceeding that of F-BAR domain alone. In living cells, the presence of the IDR delayed the recruitment of FBP17 in curvature-coupled cortical waves. Collectively, we propose that contrary to the common belief, FBP17's curvature-sensing capability largely originates from IDR, and not the F-BAR domain alone. © 2020 The Author(s)Biological Sciences; Cell Biology; Biophysics © 2020 The Author(s)
Source Title: iScience
ISSN: 25890042
DOI: 10.1016/j.isci.2020.101712
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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