Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.isci.2020.101712
DC Field | Value | |
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dc.title | Comparative Study of Curvature Sensing Mediated by F-BAR and an Intrinsically Disordered Region of FBP17 | |
dc.contributor.author | Su, M. | |
dc.contributor.author | Zhuang, Y. | |
dc.contributor.author | Miao, X. | |
dc.contributor.author | Zeng, Y. | |
dc.contributor.author | Gao, W. | |
dc.contributor.author | Zhao, W. | |
dc.contributor.author | Wu, M. | |
dc.date.accessioned | 2021-08-25T14:15:36Z | |
dc.date.available | 2021-08-25T14:15:36Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Su, M., Zhuang, Y., Miao, X., Zeng, Y., Gao, W., Zhao, W., Wu, M. (2020). Comparative Study of Curvature Sensing Mediated by F-BAR and an Intrinsically Disordered Region of FBP17. iScience 23 (11) : 101712. ScholarBank@NUS Repository. https://doi.org/10.1016/j.isci.2020.101712 | |
dc.identifier.issn | 25890042 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/199372 | |
dc.description.abstract | Membrane curvature has emerged as an intriguing physical principle underlying biological signaling and membrane trafficking. The CIP4/FBP17/Toca-1 F-BAR subfamily is unique in the BAR family because its structurally folded F-BAR domain does not contain any hydrophobic motifs that insert into membrane. Although widely assumed so, whether the banana-shaped F-BAR domain alone can sense curvature has never been experimentally demonstrated. Using a nanobar-supported lipid bilayer system, we found that the F-BAR domain of FBP17 displayed minimal curvature sensing in vitro. In comparison, an alternatively spliced intrinsically disordered region (IDR) adjacent to the F-BAR domain has the membrane curvature-sensing ability greatly exceeding that of F-BAR domain alone. In living cells, the presence of the IDR delayed the recruitment of FBP17 in curvature-coupled cortical waves. Collectively, we propose that contrary to the common belief, FBP17's curvature-sensing capability largely originates from IDR, and not the F-BAR domain alone. © 2020 The Author(s)Biological Sciences; Cell Biology; Biophysics © 2020 The Author(s) | |
dc.publisher | Elsevier Inc. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Scopus OA2020 | |
dc.subject | Biological Sciences | |
dc.subject | Biophysics | |
dc.subject | Cell Biology | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/j.isci.2020.101712 | |
dc.description.sourcetitle | iScience | |
dc.description.volume | 23 | |
dc.description.issue | 11 | |
dc.description.page | 101712 | |
Appears in Collections: | Elements Staff Publications |
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