Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-020-16135-6
Title: Par complex cluster formation mediated by phase separation
Authors: Liu, Z.
Yang, Y.
Gu, A.
Xu, J.
Mao, Y.
Lu, H.
Hu, W.
Lei, Q.-Y.
Li, Z.
Zhang, M.
Cai, Y. 
Wen, W.
Issue Date: 2020
Publisher: Nature Research
Citation: Liu, Z., Yang, Y., Gu, A., Xu, J., Mao, Y., Lu, H., Hu, W., Lei, Q.-Y., Li, Z., Zhang, M., Cai, Y., Wen, W. (2020). Par complex cluster formation mediated by phase separation. Nature Communications 11 (1) : 2266. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-020-16135-6
Rights: Attribution 4.0 International
Abstract: The evolutionarily conserved Par3/Par6/aPKC complex regulates the polarity establishment of diverse cell types and distinct polarity-driven functions. However, how the Par complex is concentrated beneath the membrane to initiate cell polarization remains unclear. Here we show that the Par complex exhibits cell cycle-dependent condensation in Drosophila neuroblasts, driven by liquid–liquid phase separation. The open conformation of Par3 undergoes autonomous phase separation likely due to its NTD-mediated oligomerization. Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation. aPKC can also be concentrated to the Par3N/Par6 condensates as a client. Interestingly, activated aPKC can disperse the Par3/Par6 condensates via phosphorylation of Par3. Perturbations of Par3/Par6 phase separation impair the establishment of apical–basal polarity during neuroblast asymmetric divisions and lead to defective lineage development. We propose that phase separation may be a common mechanism for localized cortical condensation of cell polarity complexes. © 2020, The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/199046
ISSN: 2041-1723
DOI: 10.1038/s41467-020-16135-6
Rights: Attribution 4.0 International
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