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|Title:||Par complex cluster formation mediated by phase separation||Authors:||Liu, Z.
|Issue Date:||2020||Publisher:||Nature Research||Citation:||Liu, Z., Yang, Y., Gu, A., Xu, J., Mao, Y., Lu, H., Hu, W., Lei, Q.-Y., Li, Z., Zhang, M., Cai, Y., Wen, W. (2020). Par complex cluster formation mediated by phase separation. Nature Communications 11 (1) : 2266. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-020-16135-6||Rights:||Attribution 4.0 International||Abstract:||The evolutionarily conserved Par3/Par6/aPKC complex regulates the polarity establishment of diverse cell types and distinct polarity-driven functions. However, how the Par complex is concentrated beneath the membrane to initiate cell polarization remains unclear. Here we show that the Par complex exhibits cell cycle-dependent condensation in Drosophila neuroblasts, driven by liquid–liquid phase separation. The open conformation of Par3 undergoes autonomous phase separation likely due to its NTD-mediated oligomerization. Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation. aPKC can also be concentrated to the Par3N/Par6 condensates as a client. Interestingly, activated aPKC can disperse the Par3/Par6 condensates via phosphorylation of Par3. Perturbations of Par3/Par6 phase separation impair the establishment of apical–basal polarity during neuroblast asymmetric divisions and lead to defective lineage development. We propose that phase separation may be a common mechanism for localized cortical condensation of cell polarity complexes. © 2020, The Author(s).||Source Title:||Nature Communications||URI:||https://scholarbank.nus.edu.sg/handle/10635/199046||ISSN:||2041-1723||DOI:||10.1038/s41467-020-16135-6||Rights:||Attribution 4.0 International|
|Appears in Collections:||Staff Publications|
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