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Title: High flavivirus structural plasticity demonstrated by a non-spherical morphological variant
Authors: Morrone, S.R. 
Chew, V.S.Y. 
Lim, X.-N. 
Ng, T.-S. 
Kostyuchenko, V.A. 
Zhang, S. 
Chew, P.-L. 
Lee, J. 
Tan, J.L. 
Wang, J. 
Tan, T.Y. 
Shi, J.
Screaton, G.
Morais, M.C.
Lok, S.-M. 
Issue Date: 19-Jun-2020
Publisher: Nature Research
Citation: Morrone, S.R., Chew, V.S.Y., Lim, X.-N., Ng, T.-S., Kostyuchenko, V.A., Zhang, S., Chew, P.-L., Lee, J., Tan, J.L., Wang, J., Tan, T.Y., Shi, J., Screaton, G., Morais, M.C., Lok, S.-M. (2020-06-19). High flavivirus structural plasticity demonstrated by a non-spherical morphological variant. Nature Communications 11 (1) : 3112. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Previous flavivirus (dengue and Zika viruses) studies showed largely spherical particles either with smooth or bumpy surfaces. Here, we demonstrate flavivirus particles have high structural plasticity by the induction of a non-spherical morphology at elevated temperatures: the club-shaped particle (clubSP), which contains a cylindrical tail and a disc-like head. Complex formation of DENV and ZIKV with Fab C10 stabilize the viruses allowing cryoEM structural determination to ~10 Å resolution. The caterpillar-shaped (catSP) Fab C10:ZIKV complex shows Fabs locking the E protein raft structure containing three E dimers. However, compared to the original spherical structure, the rafts have rotated relative to each other. The helical tail structure of Fab C10:DENV3 clubSP showed although the Fab locked an E protein dimer, the dimers have shifted laterally. Morphological diversity, including clubSP and the previously identified bumpy and smooth-surfaced spherical particles, may help flavivirus survival and immune evasion. © 2020, The Author(s).
Source Title: Nature Communications
ISSN: 20411723
DOI: 10.1038/s41467-020-16925-y
Rights: Attribution 4.0 International
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