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Title: C-Type Lectins Link Immunological and Reproductive Processes in Aedes aegypti
Authors: Li, H.-H.
Cai, Y. 
Li, J.-C.
Su, M.P.
Liu, W.-L.
Cheng, L.
Chou, S.-J.
Yu, G.-Y.
Wang, H.-D.
Chen, C.-H.
Keywords: Biological Sciences
Microbiology Parasite
Issue Date: 2020
Publisher: Elsevier Inc.
Citation: Li, H.-H., Cai, Y., Li, J.-C., Su, M.P., Liu, W.-L., Cheng, L., Chou, S.-J., Yu, G.-Y., Wang, H.-D., Chen, C.-H. (2020). C-Type Lectins Link Immunological and Reproductive Processes in Aedes aegypti. iScience 23 (9) : 101486. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Biological Sciences; Immunology; Microbiology Parasite © 2020Physiological trade-offs between mosquito immune response and reproductive capability can arise due to insufficient resource availability. C-type lectin family members may be involved in these processes. We established a GCTL-3?/? mutant Aedes aegypti using CRISPR/Cas9 to investigate the role of GCTL-3 in balancing the costs associated with immune responses to arboviral infection and reproduction. GCTL-3?/? mutants showed significantly reduced DENV-2 infection rate and gut commensal microbiota populations, as well as upregulated JAK/STAT, IMD, Toll, and AMPs immunological pathways. Mutants also had significantly shorter lifespans than controls and laid fewer eggs due to defective germ line development. dsRNA knock-down of Attacin and Gambicin, two targets of the AMPs pathway, partially rescued this reduction in reproductive capabilities. Upregulation of immune response following GCTL-3 knock-out therefore comes at a cost to reproductive fitness. Knock-out of other lectins may further improve our knowledge of the molecular and genetic mechanisms underlying reproduction-immunity trade-offs in mosquitoes. © 2020
Source Title: iScience
ISSN: 25890042
DOI: 10.1016/j.isci.2020.101486
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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