Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncb3456
Title: Nanoscale architecture of cadherin-based cell adhesions
Authors: Bertocchi, Cristina 
Wang, Yilin 
Ravasio, Andrea 
Hara, Yusuke 
Wu, Yao
Sailov, Talgat 
Baird, Michelle A
Davidson, Michael W
Zaidel-Bar, Ronen 
Toyama, Yusuke 
Ladoux, Benoit 
Mege, Rene-Marc
Kanchanawong, Pakorn 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
ALPHA-CATENIN
VINCULIN
ACTIN
MICROSCOPY
TENSION
COMPLEX
BINDING
CONTRACTILITY
ORGANIZATION
ASSOCIATION
Issue Date: 1-Jan-2017
Publisher: NATURE PUBLISHING GROUP
Citation: Bertocchi, Cristina, Wang, Yilin, Ravasio, Andrea, Hara, Yusuke, Wu, Yao, Sailov, Talgat, Baird, Michelle A, Davidson, Michael W, Zaidel-Bar, Ronen, Toyama, Yusuke, Ladoux, Benoit, Mege, Rene-Marc, Kanchanawong, Pakorn (2017-01-01). Nanoscale architecture of cadherin-based cell adhesions. NATURE CELL BIOLOGY 19 (1) : 28-37. ScholarBank@NUS Repository. https://doi.org/10.1038/ncb3456
Abstract: Multicellularity in animals requires dynamic maintenance of cell-cell contacts. Intercellularly ligated cadherins recruit numerous proteins to form supramolecular complexes that connect with the actin cytoskeleton and support force transmission. However, the molecular organization within such structures remains unknown. Here we mapped protein organization in cadherin-based adhesions by super-resolution microscopy, revealing a multi-compartment nanoscale architecture, with the plasma-membrane-proximal cadherin-catenin compartment segregated from the actin cytoskeletal compartment, bridged by an interface zone containing vinculin. Vinculin position is determined by α-catenin, and following activation, vinculin can extend â 1/430 nm to bridge the cadherin-catenin and actin compartments, while modulating the nanoscale positions of the actin regulators zyxin and VASP. Vinculin conformational activation requires tension and tyrosine phosphorylation, regulated by Abl kinase and PTP1B phosphatase. Such modular architecture provides a structural framework for mechanical and biochemical signal integration by vinculin, which may differentially engage cadherin-catenin complexes with the actomyosin machinery to regulate cell adhesions.
Source Title: NATURE CELL BIOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/194056
ISSN: 14657392
14764679
DOI: 10.1038/ncb3456
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