Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1008077
Title: Novel AU-rich proximal UTR sequences (APS) enhance CXCL8 synthesis upon the induction of rpS6 phosphorylation
Authors: Ang, Zhiwei 
Koean, Ricky Abdi Gunawan 
Er, Jun Zhi 
Lee, Li Ting
Tam, John Kit Chung 
Guo, Huili 
Ding, Jeak Ling 
Keywords: Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
ACTIVATED PROTEIN-KINASE
MESSENGER-RNA
GENE-EXPRESSION
CELL-LINE
S6 PHOSPHORYLATION
TNF-ALPHA
INTERLEUKIN-8
STIMULATION
SECRETION
STABILIZATION
Issue Date: 10-Apr-2019
Publisher: PUBLIC LIBRARY SCIENCE
Citation: Ang, Zhiwei, Koean, Ricky Abdi Gunawan, Er, Jun Zhi, Lee, Li Ting, Tam, John Kit Chung, Guo, Huili, Ding, Jeak Ling (2019-04-10). Novel AU-rich proximal UTR sequences (APS) enhance CXCL8 synthesis upon the induction of rpS6 phosphorylation. PLOS GENETICS 15 (4). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1008077
Abstract: The role of ribosomal protein S6 (rpS6) phosphorylation in mRNA translation remains poorly understood. Here, we reveal a potential role in modulating the translation rate of chemokine (C-X-C motif) ligand 8 (CXCL8 or Interleukin 8, IL8). We observed that more CXCL8 protein was being secreted from less CXCL8 mRNA in primary macrophages and macrophage-like HL-60 cells relative to other cell types. This correlated with an increase in CXCL8 polyribosome association, suggesting an increase in the rate of CXCL8 translation in macrophages. The cell type-specific expression levels were replicated by a CXCL8- UTR-reporter (Nanoluc reporter flanked by the 5’ and 3’ UTR of CXCL8). Mutations of the CXCL8-UTR-reporter revealed that cell type-specific expression required: 1) a 3’ UTR of at least three hundred bases; and 2) an AU base content that exceeds fifty percent in the first hundred bases of the 3’ UTR immediately after the stop codon, which we dub AU-rich proximal UTR sequences (APS). The 5’ UTR of CXCL8 enhanced expression at the protein level and conferred cell type-specific expression when paired with a 3’ UTR. A search for other APS-positive mRNAs uncovered TNF alpha induced protein 6 (TNFAIP6), another mRNA that was translationally upregulated in macrophages. The elevated translation of APS-positive mRNAs in macrophages coincided with elevated rpS6 S235/236 phosphorylation. Both were attenuated by the ERK1/2 signaling inhibitors, U0126 and AZD6244. In A549 cells, rpS6 S235/236 phosphorylation was induced by TAK1, Akt or PKA signaling. This enhanced the translation of the CXCL8-UTR-reporters. Thus, we propose that the induction of rpS6 S235/236 phosphorylation enhances the translation of mRNAs that contain APS motifs, such as CXCL8 and TNFAIP6. This may contribute to the role of macrophages as the primary producer of CXCL8, a cytokine that is essential for immune cell recruitment and activation.
Source Title: PLOS GENETICS
URI: https://scholarbank.nus.edu.sg/handle/10635/193732
ISSN: 15537404
DOI: 10.1371/journal.pgen.1008077
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