Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbm.b.34577
Title: Improving the handling properties and long-term stability of polyelectrolyte complex by freeze-drying technique for low-dose bone morphogenetic protein 2 delivery
Authors: Liu, Ling 
Lam, Wing MR 
Yang, Zheng 
Wang, Ming 
Ren, Xiafei 
Hu, Tao
Li, Jun 
Goh, James Cho-Hong 
Wong, Hee-Kit 
Keywords: Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
freeze-drying
polyelectrolyte complex
low-dose BMP-2
trehalose
spinal fusion
GROWTH-FACTOR DELIVERY
CONTROLLED-RELEASE
ALGINATE BEADS
HETEROTOPIC OSSIFICATION
INCORPORATING HEPARIN
HYALURONIC-ACID
REGENERATION
TREHALOSE
BIOACTIVITY
FORMULATION
Issue Date: 4-Feb-2020
Publisher: WILEY
Citation: Liu, Ling, Lam, Wing MR, Yang, Zheng, Wang, Ming, Ren, Xiafei, Hu, Tao, Li, Jun, Goh, James Cho-Hong, Wong, Hee-Kit (2020-02-04). Improving the handling properties and long-term stability of polyelectrolyte complex by freeze-drying technique for low-dose bone morphogenetic protein 2 delivery. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 108 (6) : 2450-2460. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.b.34577
Abstract: © 2020 Wiley Periodicals, Inc. A variety of controlled release carriers for bone morphogenetic protein 2 (BMP-2) delivery have been developed and tested in animal models. An alginate-based polyelectrolyte complex (PEC) for controlled release of low-dose BMP-2 has shown promising results in preclinical research. However, the poor handling properties and long-term stability of PEC need to be improved for translational applications. This study aimed to address these limitations of alginate-based PEC by employing a freeze-drying technique. The size and structure of freeze-dried PEC (FD-PEC) were maintained with the addition of a cryoprotectant, trehalose. The release profile of BMP-2 from FD-PEC was similar to that of freshly prepared PEC. In vitro bioactivity analysis of the released BMP-2 showed that the carrier performance of PEC was not compromised by freeze-drying up to three-month storage at room temperature. BMP-2-bound FD-PEC induced comparable bone formation to that using freshly prepared regular PEC in a rat posterolateral spinal fusion model. These results suggest that FD-PEC is capable of delivering low-dose BMP-2 and could be developed as an off-the-shelf product for translational applications. The simplicity of this preservation method provides promise for the translational application of PEC.
Source Title: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
URI: https://scholarbank.nus.edu.sg/handle/10635/184441
ISSN: 15524973
15524981
DOI: 10.1002/jbm.b.34577
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