Please use this identifier to cite or link to this item:
https://doi.org/10.1089/ten.tea.2019.0124
Title: | Synergistic Effect of NELL-1 and an Ultra-Low Dose of BMP-2 on Spinal Fusion | Authors: | Liu, L Lam, WMR Naidu, M Yang, Z Wang, M Ren, X Hu, T Kumarsing, R Ting, K Goh, JCH Wong, HK |
Keywords: | NELL-1 control release dual delivery polyelectrolyte complex spinal fusion ultra-low dose of BMP-2 Animals Biomechanical Phenomena Bone Morphogenetic Protein 2 Calcium-Binding Proteins Cell Differentiation Drug Liberation Drug Synergism Fibrinogen Osteogenesis Polyelectrolytes Rats, Sprague-Dawley Spinal Fusion Spine Swine Tissue Scaffolds X-Ray Microtomography |
Issue Date: | 1-Dec-2019 | Publisher: | Mary Ann Liebert Inc | Citation: | Liu, L, Lam, WMR, Naidu, M, Yang, Z, Wang, M, Ren, X, Hu, T, Kumarsing, R, Ting, K, Goh, JCH, Wong, HK (2019-12-01). Synergistic Effect of NELL-1 and an Ultra-Low Dose of BMP-2 on Spinal Fusion. Tissue Engineering - Part A 25 (23-24) : 1677-1689. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tea.2019.0124 | Abstract: | Copyright © 2019 Mary Ann Liebert, Inc. Bone morphogenetic protein 2 (BMP-2) is widely used in spinal fusion but it can cause adverse effects such as ectopic bone and adipose tissue in vivo. Neural epidermal growth factor like-like molecule-1 (NELL-1) has been shown to suppress BMP-2-induced adverse effects. However, no optimum carriers that control both NELL-1 and BMP-2 releases to elicit long-term bioactivity have been developed. In this study, we employed polyelectrolyte complex (PEC) as a control release carrier for NELL-1 and BMP-2. An ultra-low dose of BMP-2 synergistically functioned with NELL-1 on bone marrow mesenchymal stem cells osteogenic differentiation with greater mineralization in vitro. The osteoinductive ability of NELL-1 and an ultra-low dose of BMP-2 in PEC was investigated in rat posterolateral spinal fusion. Our results showed increased fusion rate, bone architecture, and improved bone stiffness at 8 weeks after surgery in the combination groups compared with NELL-1 or BMP-2 alone. Moreover, the formation of ectopic bone and adipose tissue was negligible in all the PEC groups. In summary, dual delivery of NELL-1 and an ultra-low dose of BMP-2 in the PEC control release carrier has greater fusion efficiency compared with BMP-2 alone and could potentially be a better alternative to the currently used BMP-2 treatments for spinal fusion. | Source Title: | Tissue Engineering - Part A | URI: | https://scholarbank.nus.edu.sg/handle/10635/184421 | ISSN: | 19373341 1937335X |
DOI: | 10.1089/ten.tea.2019.0124 |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Synergistic Effect of NELL-1 and an Ultra-Low Dose of BMP-2 on Spinal Fusion_Liu Ling.pdf | Published version | 1.21 MB | Adobe PDF | CLOSED | None |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.