Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13059-015-0643-z
Title: Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system
Authors: Hsu, L.-Y 
Harris, S.R
Chlebowicz, M.A
Lindsay, J.A
Koh, T.-H 
Krishnan, P
Tan, T.-Y 
Hon, P.-Y
Grubb, W.B
Bentley, S.D
Parkhill, J
Peacock, S.J
Holden, M.T.G
Keywords: clindamycin
cotrimoxazole
erythromycin
gentamicin
quinoline derived antiinfective agent
tetracycline
bacterial DNA
antibiotic resistance
antibiotic sensitivity
Article
bacterial colonization
bacterial transmission
controlled study
evolutionary homology
gene sequence
genetic association
genetic variability
health care system
incidence
methicillin resistant Staphylococcus aureus
nonhuman
phylogenetic tree
phylogeny
population structure
prevalence
Singapore
single nucleotide polymorphism
species dominance
bacterial genome
Bayes theorem
cross infection
DNA sequence
gene library
gene locus
genetics
hospital
human
isolation and purification
methicillin resistant Staphylococcus aureus
microbiology
molecular cloning
molecular evolution
phylogeography
population genetics
methicillin resistant Staphylococcus aureus
Bayes Theorem
Cloning, Molecular
Cross Infection
DNA, Bacterial
Evolution, Molecular
Gene Library
Genetic Loci
Genetics, Population
Genome, Bacterial
Hospitals
Humans
Methicillin-Resistant Staphylococcus aureus
Phylogeny
Phylogeography
Sequence Analysis, DNA
Singapore
Issue Date: 2015
Citation: Hsu, L.-Y, Harris, S.R, Chlebowicz, M.A, Lindsay, J.A, Koh, T.-H, Krishnan, P, Tan, T.-Y, Hon, P.-Y, Grubb, W.B, Bentley, S.D, Parkhill, J, Peacock, S.J, Holden, M.T.G (2015). Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. Genome Biology 16 (1) : 81. ScholarBank@NUS Repository. https://doi.org/10.1186/s13059-015-0643-z
Rights: Attribution 4.0 International
Abstract: Background: In the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe. Results: We investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population. Conclusions: Our results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations. @ 2015 Hsu et al.; licensee BioMed Central.
Source Title: Genome Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/183763
ISSN: 14747596
DOI: 10.1186/s13059-015-0643-z
Rights: Attribution 4.0 International
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