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https://doi.org/10.1186/s13059-015-0643-z
Title: | Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system | Authors: | Hsu, L.-Y Harris, S.R Chlebowicz, M.A Lindsay, J.A Koh, T.-H Krishnan, P Tan, T.-Y Hon, P.-Y Grubb, W.B Bentley, S.D Parkhill, J Peacock, S.J Holden, M.T.G |
Keywords: | clindamycin cotrimoxazole erythromycin gentamicin quinoline derived antiinfective agent tetracycline bacterial DNA antibiotic resistance antibiotic sensitivity Article bacterial colonization bacterial transmission controlled study evolutionary homology gene sequence genetic association genetic variability health care system incidence methicillin resistant Staphylococcus aureus nonhuman phylogenetic tree phylogeny population structure prevalence Singapore single nucleotide polymorphism species dominance bacterial genome Bayes theorem cross infection DNA sequence gene library gene locus genetics hospital human isolation and purification methicillin resistant Staphylococcus aureus microbiology molecular cloning molecular evolution phylogeography population genetics methicillin resistant Staphylococcus aureus Bayes Theorem Cloning, Molecular Cross Infection DNA, Bacterial Evolution, Molecular Gene Library Genetic Loci Genetics, Population Genome, Bacterial Hospitals Humans Methicillin-Resistant Staphylococcus aureus Phylogeny Phylogeography Sequence Analysis, DNA Singapore |
Issue Date: | 2015 | Citation: | Hsu, L.-Y, Harris, S.R, Chlebowicz, M.A, Lindsay, J.A, Koh, T.-H, Krishnan, P, Tan, T.-Y, Hon, P.-Y, Grubb, W.B, Bentley, S.D, Parkhill, J, Peacock, S.J, Holden, M.T.G (2015). Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. Genome Biology 16 (1) : 81. ScholarBank@NUS Repository. https://doi.org/10.1186/s13059-015-0643-z | Rights: | Attribution 4.0 International | Abstract: | Background: In the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe. Results: We investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population. Conclusions: Our results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations. @ 2015 Hsu et al.; licensee BioMed Central. | Source Title: | Genome Biology | URI: | https://scholarbank.nus.edu.sg/handle/10635/183763 | ISSN: | 14747596 | DOI: | 10.1186/s13059-015-0643-z | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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