Please use this identifier to cite or link to this item: https://doi.org/10.1155/2017/5839071
Title: Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton's Jelly Extracellular Matrix
Authors: Xiao, T
Guo, W
Chen, M
Hao, C
Gao, S
Huang, J 
Yuan, Z
Zhang, Y
Wang, M
Li, P
Peng, J
Wang, A
Wang, Y
Sui, X
Zhang, L
Xu, W
Lu, S
Yin, H
Yang, J
Liu, S
Guo, Q
Keywords: aggrecan
collagen type 2
glycosaminoglycan
messenger RNA
water
aggrecan
collagen
adult
animal cell
animal tissue
Article
articular cartilage
biocompatibility
biological activity
cell adhesion
cell proliferation
chondrocyte
chondrogenesis
controlled study
DNA content
extracellular matrix
female
freeze drying
human
human cell
human tissue
immunofluorescence test
in vitro study
Leporidae
microtechnology
MTT assay
nonhuman
normal human
porosity
real time polymerase chain reaction
tissue engineering
tissue section
umbilical cord
water transport
Wharton jelly
animal
articular cartilage
cell culture
cytology
extracellular matrix
metabolism
physiology
pig
pregnancy
procedures
tissue engineering
tissue scaffold
Wharton jelly
young adult
Adult
Aggrecans
Animals
Cartilage, Articular
Cell Proliferation
Cells, Cultured
Chondrocytes
Chondrogenesis
Collagen
Extracellular Matrix
Female
Glycosaminoglycans
Humans
Porosity
Pregnancy
Swine
Tissue Engineering
Tissue Scaffolds
Umbilical Cord
Wharton Jelly
Young Adult
Issue Date: 2017
Publisher: Hindawi
Citation: Xiao, T, Guo, W, Chen, M, Hao, C, Gao, S, Huang, J, Yuan, Z, Zhang, Y, Wang, M, Li, P, Peng, J, Wang, A, Wang, Y, Sui, X, Zhang, L, Xu, W, Lu, S, Yin, H, Yang, J, Liu, S, Guo, Q (2017). Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton's Jelly Extracellular Matrix. BioMed Research International 2017 : 5839071. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/5839071
Rights: Attribution 4.0 International
Abstract: The scaffold is a key element in cartilage tissue engineering. The components of Wharton's jelly are similar to those of articular cartilage and it also contains some chondrogenic growth factors, such as insulin-like growth factor I and transforming growth factor-?. We fabricated a tissue-engineered cartilage scaffold derived from Wharton's jelly extracellular matrix (WJECM) and compared it with a scaffold derived from articular cartilage ECM (ACECM) using freeze-drying. The results demonstrated that both WJECM and ACECM scaffolds possessed favorable pore sizes and porosities; moreover, they showed good water uptake ratios and compressive moduli. Histological staining confirmed that the WJECM and ACECM scaffolds contained similar ECM. Moreover, both scaffolds showed good cellular adherence, bioactivity, and biocompatibility. MTT and DNA content assessments confirmed that the ACECM scaffold tended to be more beneficial for improving cell proliferation than the WJECM scaffold. However, RT-qPCR results demonstrated that the WJECM scaffold was more favorable to enhance cellular chondrogenesis than the ACECM scaffold, showing more collagen II and aggrecan mRNA expression. These results were confirmed indirectly by glycosaminoglycan and collagen content assessments and partially confirmed by histology and immunofluorescent staining. In conclusion, these results suggest that a WJECM scaffold may be favorable for future cartilage tissue engineering. © 2017 Tongguang Xiao et al.
Source Title: BioMed Research International
URI: https://scholarbank.nus.edu.sg/handle/10635/183554
ISSN: 2314-6133
DOI: 10.1155/2017/5839071
Rights: Attribution 4.0 International
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