Please use this identifier to cite or link to this item: https://doi.org/10.18632/oncotarget.13502
Title: High-risk HPV genotypes and P16INK4a expression in a cohort of head and neck squamous cell carcinoma patients in Singapore
Authors: Tan, L.S.Y
Fredrik, P
Ker, L
Yu, F.G 
Wang, D.Y 
Goh, B.C 
Loh, K.S 
Lim, C.M 
Keywords: cyclin dependent kinase inhibitor 2A
DNA
protein p16
virus DNA
cyclin dependent kinase inhibitor 2A
adult
alcohol consumption
Article
cancer prognosis
cancer risk
cancer specific survival
cancer tissue
clinical outcome
cohort analysis
controlled study
female
genotype
head and neck squamous cell carcinoma
human
human cell
Human papillomavirus type 16
Human papillomavirus type 18
Human papillomavirus type 31
Human papillomavirus type 45
Human papillomavirus type 56
Human papillomavirus type 68
human tissue
immunohistochemistry
intermethod comparison
major clinical study
male
nonhuman
oropharynx squamous cell carcinoma
overall survival
polymerase chain reaction
prevalence
protein expression
reliability
sensitivity and specificity
Singapore
smoking
survival rate
survival time
virus carcinogenesis
virus detection
virus identification
Wart virus
Asian continental ancestry group
biosynthesis
ethnology
genetics
genotype
head and neck tumor
host pathogen interaction
Kaplan Meier method
metabolism
middle aged
oropharynx tumor
Papillomaviridae
papillomavirus infection
squamous cell carcinoma
virology
young adult
Adult
Asian Continental Ancestry Group
Carcinoma, Squamous Cell
Cohort Studies
Cyclin-Dependent Kinase Inhibitor p16
Female
Genotype
Head and Neck Neoplasms
Host-Pathogen Interactions
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Oropharyngeal Neoplasms
Papillomaviridae
Papillomavirus Infections
Singapore
Young Adult
Issue Date: 2016
Publisher: Impact Journals LLC
Citation: Tan, L.S.Y, Fredrik, P, Ker, L, Yu, F.G, Wang, D.Y, Goh, B.C, Loh, K.S, Lim, C.M (2016). High-risk HPV genotypes and P16INK4a expression in a cohort of head and neck squamous cell carcinoma patients in Singapore. Oncotarget 7 (52) : 86730-86739. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.13502
Rights: Attribution 4.0 International
Abstract: Human papillomavirus (HPV), especially HPV16 genotype, is associated with oropharyngeal squamous cell carcinoma (OPSCC). We aim to determine the prevalence and characterize the high-risk (HR)-HPV genotypes in head and neck SCC (HNSCC) in a South-East Asian multi-ethnic society in Singapore and examine its prognostic significance. 159 HNSCC archival tissue samples were retrieved and tumour DNA was screened for 18 HR-HPV genotypes using a PCR-based assay (Qiagen, digene HPV genotyping RH test). P16 protein overexpression was identified using immunohistochemistry (IHC). Statistical correlation between clinical outcomes were performed between HPV-positive and negative HNSCC patients. Six HR-HPVs (HPV16, 18, 31, 45, 56, 68) were detected in 90.6% of HNSCC; and 79.9% had multiple HPV genotypes detected. HPV31 and HPV45 were the most prevalent (79.2% and 87.4%, respectively); and HPV16 was predominantly found in OPSCC (p < 0.001). HPV-DNA PCR assay yielded a high sensitivity (96%) but low specificity (11%) when compared to p16 immunohistochemistry as the reference standard. P16-positive HNSCC was predominantly observed in OPSCC (73.7%; p = 0.005); and p16-positive OPSCC exhibited improved overall survival compared to p16-negative OPSCC (p = 0.022). Similarly, smoking and alcohol consumption were poor prognostic factors of overall survival (p = 0.007; p = 0.01) in OPSCC patients. HR-HPVs were identified in 90.6% of HNSCC patients using the HPV-DNA PCR assay. This test had a poor specificity when compared to p16 IHC; making it an unreliable detection technique in selecting patients for radiation dose de-escalation treatment protocol. P16-positive tumor was predominantly found in the oropharynx these patients demonstrated better overall survival than those with p16-negative OPSCC.
Source Title: Oncotarget
URI: https://scholarbank.nus.edu.sg/handle/10635/182519
ISSN: 1949-2553
DOI: 10.18632/oncotarget.13502
Rights: Attribution 4.0 International
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