Please use this identifier to cite or link to this item: https://doi.org/10.18632/oncotarget.13502
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dc.titleHigh-risk HPV genotypes and P16INK4a expression in a cohort of head and neck squamous cell carcinoma patients in Singapore
dc.contributor.authorTan, L.S.Y
dc.contributor.authorFredrik, P
dc.contributor.authorKer, L
dc.contributor.authorYu, F.G
dc.contributor.authorWang, D.Y
dc.contributor.authorGoh, B.C
dc.contributor.authorLoh, K.S
dc.contributor.authorLim, C.M
dc.date.accessioned2020-10-31T11:44:10Z
dc.date.available2020-10-31T11:44:10Z
dc.date.issued2016
dc.identifier.citationTan, L.S.Y, Fredrik, P, Ker, L, Yu, F.G, Wang, D.Y, Goh, B.C, Loh, K.S, Lim, C.M (2016). High-risk HPV genotypes and P16INK4a expression in a cohort of head and neck squamous cell carcinoma patients in Singapore. Oncotarget 7 (52) : 86730-86739. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.13502
dc.identifier.issn1949-2553
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/182519
dc.description.abstractHuman papillomavirus (HPV), especially HPV16 genotype, is associated with oropharyngeal squamous cell carcinoma (OPSCC). We aim to determine the prevalence and characterize the high-risk (HR)-HPV genotypes in head and neck SCC (HNSCC) in a South-East Asian multi-ethnic society in Singapore and examine its prognostic significance. 159 HNSCC archival tissue samples were retrieved and tumour DNA was screened for 18 HR-HPV genotypes using a PCR-based assay (Qiagen, digene HPV genotyping RH test). P16 protein overexpression was identified using immunohistochemistry (IHC). Statistical correlation between clinical outcomes were performed between HPV-positive and negative HNSCC patients. Six HR-HPVs (HPV16, 18, 31, 45, 56, 68) were detected in 90.6% of HNSCC; and 79.9% had multiple HPV genotypes detected. HPV31 and HPV45 were the most prevalent (79.2% and 87.4%, respectively); and HPV16 was predominantly found in OPSCC (p < 0.001). HPV-DNA PCR assay yielded a high sensitivity (96%) but low specificity (11%) when compared to p16 immunohistochemistry as the reference standard. P16-positive HNSCC was predominantly observed in OPSCC (73.7%; p = 0.005); and p16-positive OPSCC exhibited improved overall survival compared to p16-negative OPSCC (p = 0.022). Similarly, smoking and alcohol consumption were poor prognostic factors of overall survival (p = 0.007; p = 0.01) in OPSCC patients. HR-HPVs were identified in 90.6% of HNSCC patients using the HPV-DNA PCR assay. This test had a poor specificity when compared to p16 IHC; making it an unreliable detection technique in selecting patients for radiation dose de-escalation treatment protocol. P16-positive tumor was predominantly found in the oropharynx these patients demonstrated better overall survival than those with p16-negative OPSCC.
dc.publisherImpact Journals LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectcyclin dependent kinase inhibitor 2A
dc.subjectDNA
dc.subjectprotein p16
dc.subjectvirus DNA
dc.subjectcyclin dependent kinase inhibitor 2A
dc.subjectadult
dc.subjectalcohol consumption
dc.subjectArticle
dc.subjectcancer prognosis
dc.subjectcancer risk
dc.subjectcancer specific survival
dc.subjectcancer tissue
dc.subjectclinical outcome
dc.subjectcohort analysis
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectgenotype
dc.subjecthead and neck squamous cell carcinoma
dc.subjecthuman
dc.subjecthuman cell
dc.subjectHuman papillomavirus type 16
dc.subjectHuman papillomavirus type 18
dc.subjectHuman papillomavirus type 31
dc.subjectHuman papillomavirus type 45
dc.subjectHuman papillomavirus type 56
dc.subjectHuman papillomavirus type 68
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectintermethod comparison
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectnonhuman
dc.subjectoropharynx squamous cell carcinoma
dc.subjectoverall survival
dc.subjectpolymerase chain reaction
dc.subjectprevalence
dc.subjectprotein expression
dc.subjectreliability
dc.subjectsensitivity and specificity
dc.subjectSingapore
dc.subjectsmoking
dc.subjectsurvival rate
dc.subjectsurvival time
dc.subjectvirus carcinogenesis
dc.subjectvirus detection
dc.subjectvirus identification
dc.subjectWart virus
dc.subjectAsian continental ancestry group
dc.subjectbiosynthesis
dc.subjectethnology
dc.subjectgenetics
dc.subjectgenotype
dc.subjecthead and neck tumor
dc.subjecthost pathogen interaction
dc.subjectKaplan Meier method
dc.subjectmetabolism
dc.subjectmiddle aged
dc.subjectoropharynx tumor
dc.subjectPapillomaviridae
dc.subjectpapillomavirus infection
dc.subjectsquamous cell carcinoma
dc.subjectvirology
dc.subjectyoung adult
dc.subjectAdult
dc.subjectAsian Continental Ancestry Group
dc.subjectCarcinoma, Squamous Cell
dc.subjectCohort Studies
dc.subjectCyclin-Dependent Kinase Inhibitor p16
dc.subjectFemale
dc.subjectGenotype
dc.subjectHead and Neck Neoplasms
dc.subjectHost-Pathogen Interactions
dc.subjectHumans
dc.subjectKaplan-Meier Estimate
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectOropharyngeal Neoplasms
dc.subjectPapillomaviridae
dc.subjectPapillomavirus Infections
dc.subjectSingapore
dc.subjectYoung Adult
dc.typeArticle
dc.contributor.departmentDEPT OF OTOLARYNGOLOGY
dc.contributor.departmentDEPT OF PHARMACOLOGY
dc.description.doi10.18632/oncotarget.13502
dc.description.sourcetitleOncotarget
dc.description.volume7
dc.description.issue52
dc.description.page86730-86739
dc.published.statepublished
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