Please use this identifier to cite or link to this item: https://doi.org/10.1111/jcmm.12351
Title: iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium
Authors: Miao, Q
Shim, W 
Tee, N
Lim, S.Y
Chung, Y.Y
Myu Mia Ja, K.P
Ooi, T.H
Tan, G
Kong, G
Wei, H 
Lim, C.H
Sin, Y.K 
Wong, P
Keywords: animal
cytology
disease model
echocardiography
echography
female
heart infarction
heart muscle
heart ventricle remodeling
human
mesenchymal stem cell transplantation
mesenchymal stroma cell
mouse
pathology
pathophysiology
physiology
pluripotent stem cell
SCID mouse
transplantation
Animals
Disease Models, Animal
Echocardiography
Female
Humans
Induced Pluripotent Stem Cells
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells
Mice
Mice, SCID
Myocardial Infarction
Myocardium
Ventricular Remodeling
Issue Date: 2014
Citation: Miao, Q, Shim, W, Tee, N, Lim, S.Y, Chung, Y.Y, Myu Mia Ja, K.P, Ooi, T.H, Tan, G, Kong, G, Wei, H, Lim, C.H, Sin, Y.K, Wong, P (2014). iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium. Journal of Cellular and Molecular Medicine 18 (8) : 1644-1654. ScholarBank@NUS Repository. https://doi.org/10.1111/jcmm.12351
Rights: Attribution 4.0 International
Abstract: We investigated global and regional effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) in infarcted myocardium. Acute myocardial infarction (MI) was induced by ligation of left coronary artery of severe combined immunodeficient mice before 2 × 10 5 iMSCs or cell-free saline were injected into peri-infarcted anterior free wall. Sham-operated animals received no injection. Global and regional myocardial function was assessed serially at 1-week and 8-week by segmental strain analysis by using two dimensional (2D) speckle tracking echocardiography. Early myocardial remodelling was observed at 1-week and persisted to 8-week with global contractility of ejection fraction and fractional area change in saline- (32.96 ± 14.23%; 21.50 ± 10.07%) and iMSC-injected (32.95 ± 10.31%; 21.00 ± 7.11%) groups significantly depressed as compared to sham control (51.17 ± 11.69%, P < 0.05; 34.86 ± 9.82%, P < 0.05). However, myocardial dilatation was observed in saline-injected animals (4.40 ± 0.62 mm, P < 0.05), but not iMSCs (4.29 ± 0.57 mm), when compared to sham control (3.74 ± 0.32 mm). Furthermore, strain analysis showed significant improved basal anterior wall strain (28.86 ± 8.16%, P < 0.05) in the iMSC group, but not saline-injected (15.81 ± 13.92%), when compared to sham control (22.18 ± 4.13%). This was corroborated by multi-segments deterioration of radial strain only in saline-injected (21.50 ± 5.31%, P < 0.05), but not iMSC (25.67 ± 12.53%), when compared to sham control (34.88 ± 5.77%). Improvements of the myocardial strain coincided with the presence of interconnecting telocytes in interstitial space of the infarcted anterior segment of the heart. Our results show that localized injection of iMSCs alleviates ventricular remodelling, sustains global and regional myocardial strain by paracrine-driven effect on neoangiogenesis and myocardial deformation/compliance via parenchymal and interstitial cell interactions in the infarcted myocardium. © 2014 The Authors.
Source Title: Journal of Cellular and Molecular Medicine
URI: https://scholarbank.nus.edu.sg/handle/10635/181523
ISSN: 15821838
DOI: 10.1111/jcmm.12351
Rights: Attribution 4.0 International
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