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Title: A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
Authors: Song, K.-Y
Zhao, H
Jiang, Z.-Y
Li, X.-F
Deng, Y.-Q
Jiang, T
Zhu, S.-Y
Shi, P.-Y 
Zhang, B
Zhang, F.-C
Qin, E.-D
Qin, C.-F
Keywords: Animals
Antibodies, Blocking
Antibodies, Neutralizing
Antibodies, Viral
Cell Line
Dengue Virus
Genes, Reporter
Luciferases, Renilla
Reproducibility of Results
Issue Date: 2014
Citation: Song, K.-Y, Zhao, H, Jiang, Z.-Y, Li, X.-F, Deng, Y.-Q, Jiang, T, Zhu, S.-Y, Shi, P.-Y, Zhang, B, Zhang, F.-C, Qin, E.-D, Qin, C.-F (2014). A novel reporter system for neutralizing and enhancing antibody assay against dengue virus. BMC Microbiology 14 (1) : 44. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Abstract. Background: Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies. Results: In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable Renilla luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals. Conclusions: This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies. © 2014 Song et al.; licensee BioMed Central Ltd.
Source Title: BMC Microbiology
ISSN: 14712180
DOI: 10.1186/1471-2180-14-44
Rights: Attribution 4.0 International
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