Please use this identifier to cite or link to this item:
https://doi.org/10.1007/s12017-018-8484-z
Title: | Vitamin D3 Supplementation Reduces Subsequent Brain Injury and Inflammation Associated with Ischemic Stroke | Authors: | Evans, M.A Kim, H.A Ling, Y.H Uong, S Vinh, A De Silva, T.M Arumugam, T.V Clarkson, A.N Zosky, G.R Drummond, G.R Broughton, B.R.S Sobey, C.G |
Keywords: | alcohol calcitriol interleukin 1beta interleukin 23p19 interleukin 6 propylene glycol reduced nicotinamide adenine dinucleotide phosphate oxidase 2 retinoid related orphan receptor gamma sterile water transcription factor FOXP3 transforming growth factor beta autacoid colecalciferol cytokine forkhead transcription factor Foxp3 protein, mouse nerve protein neuroprotective agent retinoid related orphan receptor gamma Rorc protein, mouse animal experiment animal tissue antiinflammatory activity Article brain infarction size brain injury brain ischemia controlled study disease association gamma delta T lymphocyte immune response immunocompetent cell inflammation lymphocyte count male middle cerebral artery occlusion mouse neutrophil count nonhuman priority journal protein expression regulatory T lymphocyte reperfusion injury Th17 cell vitamin supplementation animal biosynthesis brain C57BL mouse cerebral artery disease drug effect gene expression regulation genetics inflammation macrophage metabolism microglia motor activity neutrophil chemotaxis pathology pathophysiology T lymphocyte subpopulation Animals Brain Cholecalciferol Cytokines Forkhead Transcription Factors Gene Expression Regulation Infarction, Middle Cerebral Artery Inflammation Inflammation Mediators Macrophages Male Mice, Inbred C57BL Microglia Motor Activity Nerve Tissue Proteins Neuroprotective Agents Neutrophil Infiltration Nuclear Receptor Subfamily 1, Group F, Member 3 T-Lymphocyte Subsets |
Issue Date: | 2018 | Citation: | Evans, M.A, Kim, H.A, Ling, Y.H, Uong, S, Vinh, A, De Silva, T.M, Arumugam, T.V, Clarkson, A.N, Zosky, G.R, Drummond, G.R, Broughton, B.R.S, Sobey, C.G (2018). Vitamin D3 Supplementation Reduces Subsequent Brain Injury and Inflammation Associated with Ischemic Stroke. NeuroMolecular Medicine 20 (1) : 147-159. ScholarBank@NUS Repository. https://doi.org/10.1007/s12017-018-8484-z | Rights: | Attribution 4.0 International | Abstract: | Acute inflammation can exacerbate brain injury after ischemic stroke. Beyond its well-characterized role in calcium metabolism, it is becoming increasingly appreciated that the active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25-VitD3), has potent immunomodulatory properties. Here, we aimed to determine whether 1,25-VitD3 supplementation could reduce subsequent brain injury and associated inflammation after ischemic stroke. Male C57Bl6 mice were randomly assigned to be administered either 1,25-VitD3 (100 ng/kg/day) or vehicle i.p. for 5 day prior to stroke. Stroke was induced via middle cerebral artery occlusion for 1 h followed by 23 h reperfusion. At 24 h post-stroke, we assessed infarct volume, functional deficit, expression of inflammatory mediators and numbers of infiltrating immune cells. Supplementation with 1,25-VitD3 reduced infarct volume by 50% compared to vehicle. Expression of pro-inflammatory mediators IL-6, IL-1β, IL-23a, TGF-β and NADPH oxidase-2 was reduced in brains of mice that received 1,25-VitD3 versus vehicle. Brain expression of the T regulatory cell marker, Foxp3, was higher in mice supplemented with 1,25-VitD3 versus vehicle, while expression of the transcription factor, ROR-γ, was decreased, suggestive of a reduced Th17/γδ T cell response. Immunohistochemistry indicated that similar numbers of neutrophils and T cells were present in the ischemic hemispheres of 1,25-VitD3- and vehicle-supplemented mice. At this early time point, there were also no differences in the impairment of motor function. These data indicate that prior administration of exogenous vitamin D, even to vitamin D-replete mice, can attenuate infarct development and exert acute anti-inflammatory actions in the ischemic and reperfused brain. © 2018, The Author(s). | Source Title: | NeuroMolecular Medicine | URI: | https://scholarbank.nus.edu.sg/handle/10635/181211 | ISSN: | 15351084 | DOI: | 10.1007/s12017-018-8484-z | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1007_s12017-018-8484-z.pdf | 1.04 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License