Please use this identifier to cite or link to this item: https://doi.org/10.3389/fncel.2018.00059
Title: Mechanical strain alters cellular and nuclear dynamics at early stages of oligodendrocyte differentiation
Authors: Makhija, E
Jagielska, A
Zhu, L
Bost, A.C
Ong, W
Chew, S.Y
Shivashankar, G.V 
Van Vliet, K.J
Keywords: animal cell
Article
cell migration
controlled study
correlation coefficient
genetic transfection
live cell imaging
mechanotransduction
microtubule
nerve cell differentiation
nonhuman
oligodendrocyte culture
oligodendrocyte precursor cell
rat
time lapse imaging
Issue Date: 2018
Citation: Makhija, E, Jagielska, A, Zhu, L, Bost, A.C, Ong, W, Chew, S.Y, Shivashankar, G.V, Van Vliet, K.J (2018). Mechanical strain alters cellular and nuclear dynamics at early stages of oligodendrocyte differentiation. Frontiers in Cellular Neuroscience 12 : 59. ScholarBank@NUS Repository. https://doi.org/10.3389/fncel.2018.00059
Rights: Attribution 4.0 International
Abstract: Mechanical and physical stimuli including material stiffness and topography or applied mechanical strain have been demonstrated to modulate differentiation of glial progenitor and neural stem cells. Recent studies probing such mechanotransduction in oligodendrocytes have focused chiefly on the biomolecular components. However, the cell-level biophysical changes associated with such responses remain largely unknown. Here, we explored mechanotransduction in oligodendrocyte progenitor cells (OPCs) during the first 48h of differentiation induction by quantifying the biophysical state in terms of nuclear dynamics, cytoskeleton organization, and cell migration. We compared these mechanophenotypic changes in OPCs exposed to both chemical cues (differentiation factors) and mechanical cues (static tensile strain of 10%) with those exposed to only those chemical cues. We observed that mechanical strain significantly hastened the dampening of nuclear fluctuations and decreased OPC migration, consistent with the progression of differentiation. Those biophysical changes were accompanied by increased production of the intracellular microtubule network. These observations provide insights into mechanisms by which mechanical strain of physiological magnitude could promote differentiation of progenitor cells to oligodendrocytes via inducing intracellular biophysical responses over hours to days post induction. © 2018 Makhija, Jagielska, Zhu, Bost, Ong, Chew, Shivashankar and Van Vliet.
Source Title: Frontiers in Cellular Neuroscience
URI: https://scholarbank.nus.edu.sg/handle/10635/181210
ISSN: 16625102
DOI: 10.3389/fncel.2018.00059
Rights: Attribution 4.0 International
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