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Title: RanGAP-mediated nucleocytoplasmic transport of Prospero regulates neural stem cell lifespan in Drosophila larval central brain
Authors: Wu, D
Wu, L
An, H
Bao, H
Guo, P
Zhang, B
Zheng, H
Zhang, F
Ge, W
Cai, Y 
Xi, Y
Yang, X
Keywords: caspase 3
dextro phenylalanylcysteinyltyrosyl dextro tryptophylarginylthreonylpenicillaminylthreoninamide 2,7 disulfide
guanosine triphosphatase activating protein
rangap protein
transcription factor
transcription factor Prospero
unclassified drug
animal cell
animal tissue
brain development
cell nucleus membrane
cell survival
cellular distribution
controlled study
CRISPR-CAS9 system
embryo development
genetic background
larval stage
nervous system development
neural stem cell
nonsense mutation
nuclear export signal
nuclear localization signal
nucleocytoplasmic transport
null allele
priority journal
RNA interference
sequence analysis
Issue Date: 2019
Citation: Wu, D, Wu, L, An, H, Bao, H, Guo, P, Zhang, B, Zheng, H, Zhang, F, Ge, W, Cai, Y, Xi, Y, Yang, X (2019). RanGAP-mediated nucleocytoplasmic transport of Prospero regulates neural stem cell lifespan in Drosophila larval central brain. Aging Cell 18 (1) : e12854. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: By the end of neurogenesis in Drosophila pupal brain neuroblasts (NBs), nuclear Prospero (Pros) triggers cell cycle exit and terminates NB lifespan. Here, we reveal that in larval brain NBs, an intrinsic mechanism facilitates import and export of Pros across the nuclear envelope via a Ran-mediated nucleocytoplasmic transport system. In rangap mutants, the export of Pros from the nucleus to cytoplasm is impaired and the nucleocytoplasmic transport of Pros becomes one-way traffic, causing an early accumulation of Pros in the nuclei of the larval central brain NBs. This nuclear Pros retention initiates NB cell cycle exit and leads to a premature decrease of total NB numbers. Our data indicate that RanGAP plays a crucial role in this intrinsic mechanism that controls NB lifespan during neurogenesis. Our study may provide insights into understanding the lifespan of neural stem cells during neurogenesis in other organisms. © 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
Source Title: Aging Cell
ISSN: 14749718
DOI: 10.1111/acel.12854
Rights: Attribution 4.0 International
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