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Title: Different Subpopulations of Developing Thymocytes are Associated With Adherent (Macrophage) or Nonadherent (Dendritic) Thymic Rosettes
Authors: Shortman, K 
Vremec, D
Keywords: Antigens, Differentiation, T Lymphocyte
CD11b antigen
CD3 antigen
CD4 antigen
CD8 antigen
lymphocyte antigen receptor
Macrophage 1 Antigen
Receptors, Antigen, T Cell
T lymphocyte antigen
flow cytometry
fluorescent antibody technique
mouse strain
rosette formation
T lymphocyte subpopulation
Antigens, CD3
Antigens, CD4
Antigens, CD8
Antigens, Differentiation, T-Lymphocyte
Flow Cytometry
Fluorescent Antibody Technique
Macrophage-1 Antigen
Mice, Inbred C57BL
Mice, Inbred CBA
Receptors, Antigen, T-Cell
Rosette Formation
Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S.
T-Lymphocyte Subsets
Thymus Gland
Issue Date: 1991
Citation: Shortman, K, Vremec, D (1991). Different Subpopulations of Developing Thymocytes are Associated With Adherent (Macrophage) or Nonadherent (Dendritic) Thymic Rosettes. Developmental Immunology 1 (3) : 225-235. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Thymic rosettes (ROS), structures consisting of thymic lymphoid cells attached to a central stromal cell, were isolated from mouse thymus by collagenase digestion and unit-gravity elutriation. The ROS were then separated into those where the stromal cells were either macrophage-like (M-ROS) or dendritic cell-like (D-ROS), on the basis of the differences in adherence properties or in the level of MAC-1 surface antigen. The ROS were then dissociated and the thymocyte content analyzed by immunofluorescent staining and flow cytometry. M-ROS and D-ROS differed in thymocyte composition, although the major component of both was the CD4+CD8+ cortical thymocyte. D-ROS were enriched in thymocytes expressing high levels of surface T-cell antigen receptor (TcR) and the associated CD3 complex, and these included both CD4+CD8-CD3++ and CD4-CD8+CD3++ mature thymocytes. M-ROS were enriched in CD4-CD8++ thymocytes and had a reduced content of thymocytes expressing high TcR-CD3 levels; they nevertheless contained some mature thymocytes, but only of the CD4+CD8-CD3++ category. Several lines of evidence indicated that the mature thymocytes in ROS were cells recently formed in the cortex, and were not from the medullary pool. ROS- associated mature thymocytes expressed lower levels of H-2K than free, mature thymocytes. The CD4+CD8+CD3++ subpopulation, believed to be a developmental intermediate between cortical thymocytes and mature T cells, was present in both ROS populations. Further, late intermediates leading to both mature T-cell categories were evident in D-ROS, but only those leading to CD4+CD8-CD3++ T cells were evident in M- ROS. The results are compatible with a role for ROS in TcR-specificity selection and in the final maturation steps in the thymic cortex. © 1991, Harwood Academic Publishers GmbH.
Source Title: Developmental Immunology
ISSN: 10446672
DOI: 10.1155/1991/49025
Rights: Attribution 4.0 International
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