Please use this identifier to cite or link to this item:
Title: Cytotoxic T lymphocytes recognize an HLA-A2-restricted epitope within the hepatitis B virus nucleocapsid antigen
Authors: Penna, A
Chisari, F.V
Bertoletti, A 
Missale, G
Fowler, P
Giuberti, T
Fiaccadori, F
Ferrari, C
Keywords: epitope
hepatitis b core antigen
hepatitis b(e) antigen
HLA A2 antigen
synthetic peptide
clinical article
controlled study
cytotoxic t lymphocyte
hepatitis b virus
human cell
priority journal
Alanine Transaminase
Hepatitis B
Hepatitis B Core Antigens
Hepatitis B e Antigens
HLA-A2 Antigen
Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S.
T-Lymphocytes, Cytotoxic
Issue Date: 1991
Citation: Penna, A, Chisari, F.V, Bertoletti, A, Missale, G, Fowler, P, Giuberti, T, Fiaccadori, F, Ferrari, C (1991). Cytotoxic T lymphocytes recognize an HLA-A2-restricted epitope within the hepatitis B virus nucleocapsid antigen. Journal of Experimental Medicine 174 (6) : 1565-1570. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The absence of readily manipulable experimental systems to study the cytotoxic T lymphocyte(CTL) response against hepatitis B virus (HBV) antigens has thus far precluded a definitive demonstration of the role played by this response in the pathogenesis of liver cell injury and viral clearance during HBV infection. To circumvent the problem that HBV infection of human cells in vitro for production of stimulator/target systems for CTL analysis is not feasible, a panel of 22 overlapping synthetic peptides covering the entire amino acid sequence of the HBV core (HBcAg) and e (HBeAg) antigens were used to induce and to analyze the HBV nucleocapsid-specific CTL response in nine patients with acute hepatitis B, six patients with chronic active hepatitis B, and eight normal controls. By using this approach, we have identified an HLA-A2-restricted CTL epitope, located within the NH2-terminal region of the HBV core molecule, which is shared with the e antigen and is readily recognized by peripheral blood mononudear cells from patients with self-limited acute hepatitis B but less efficiently in chronic HBV infection. Our study provides the first direct evidence of HLA class I-restricted T cell cytotoxicity against HBV in humans. Furthermore, the different response in HBV-infected subjects who successfully clear the virus (acute patients) in comparison with patients who do not succeed (chronic patients) suggests a pathogenetic role for this CTL activity in the clearance of HBV infection. © 1991, Rockefeller University Press., All rights reserved.
Source Title: Journal of Experimental Medicine
ISSN: 00221007
DOI: 10.1084/jem.174.6.1565
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1084_jem_174_6_1565.pdf538.46 kBAdobe PDF




checked on Nov 28, 2020

Page view(s)

checked on Nov 26, 2020

Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons