Please use this identifier to cite or link to this item: https://doi.org/10.1155/1993/43482
Title: Characterization of Thymic Nurse-Cell Lymphocytes, Using an Improved Procedure for Nurse-Cell Isolation
Authors: Lahoud, M
Vremec, D
Boyd, R.L
Shortman, K 
Keywords: biological marker
CD3 antigen
CD4 antigen
CD8 antigen
animal
article
cell separation
cytology
female
flow cytometry
methodology
mouse
mouse strain
phenotype
physiology
T lymphocyte
T lymphocyte subpopulation
thymus
Animal
Antigens, CD3
Antigens, CD4
Antigens, CD8
Biological Markers
CD4-Positive T-Lymphocytes
Cell Separation
Female
Flow Cytometry
Mice
Mice, Inbred CBA
Phenotype
Support, Non-U.S. Gov't
T-Lymphocyte Subsets
T-Lymphocytes
Thymus Gland
Issue Date: 1993
Citation: Lahoud, M, Vremec, D, Boyd, R.L, Shortman, K (1993). Characterization of Thymic Nurse-Cell Lymphocytes, Using an Improved Procedure for Nurse-Cell Isolation. Developmental Immunology 3 (2) : 103-112. ScholarBank@NUS Repository. https://doi.org/10.1155/1993/43482
Rights: Attribution 4.0 International
Abstract: Thymic nurse cells (TNC), multicellular complexes consisting of lymphoid cells enclosed within cortical epithelial cells, were isolated from mouse thymus by a modified procedure allowing immunofluorescent labeling and flow cytometric analysis of their lymphoid contents (TNC-L). Collagenase was the only protease used for tissue digestion, to ensure that surface antigen markers remained intact. Zonal unit-gravity elutriation was used to enrich the TNC on the basis of their high sedimentation rate, followed by immunomagnetic bead depletion to remove residual mononuclear cell contaminants and a density separation to remove debris. The TNC-L were then released from inside TNC by a short period of culture. The measured contamination of TNC-L with exogenous thymocytes was around 0.5%. Three-color immunofluorescent labeling revealed that TNC-L included, as well as a majority of immature CD4+8+3low thymocytes, about 12% of apparently mature CD4+8-3high and CD4~8+3high thymocytes. TNC are located in the cortex, where mature cells are rare; the occurrence of mature phenotype cells within these structures suggests that they represent a microenvironment for the selection and generation of mature T cells. © 1993, Harwood Academic Publishers GmbH.
Source Title: Developmental Immunology
URI: https://scholarbank.nus.edu.sg/handle/10635/181148
ISSN: 10446672
DOI: 10.1155/1993/43482
Rights: Attribution 4.0 International
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