Please use this identifier to cite or link to this item: https://doi.org/10.1155/2003/479764
Title: Chemical characterization of a new carbohydrate metabolite in the vitreous of Black Moor goldfish by liquid chromatography-electrospray mass spectrometry and nuclear magnetic resonance
Authors: Zhou, L 
Juneau, G
Xiong, X
Lai, H.W.L
Yew, D.T
Lam, K.-W
Keywords: Light absorption
Liquid chromatography
Mass spectrometry
Metabolites
Nuclear magnetic resonance spectroscopy
Structure (composition)
Lactone rings
Carbohydrates
acetonitrile
carbon
lactone
trifluoroacetic acid
water
aqueous humor
article
carbohydrate analysis
chemical structure
concentration (parameters)
goldfish
high performance liquid chromatography
light absorption
mammal
mass spectrometry
nonhuman
nuclear magnetic resonance
serum
species
vitreous body
Issue Date: 2003
Publisher: IOS Press
Citation: Zhou, L, Juneau, G, Xiong, X, Lai, H.W.L, Yew, D.T, Lam, K.-W (2003). Chemical characterization of a new carbohydrate metabolite in the vitreous of Black Moor goldfish by liquid chromatography-electrospray mass spectrometry and nuclear magnetic resonance. Spectroscopy 17 (4) : 715-724. ScholarBank@NUS Repository. https://doi.org/10.1155/2003/479764
Rights: Attribution 4.0 International
Abstract: Our recent report has described the occurrence of a remarkably high amount of an unknown compound with light absorption maximum at 290 nm in the aqueous humor of Black Moor goldfish. The absorption maximum is shifted to 270 nm in 0.01% trifluroacetic acid. The concentration of this compound was very low in the eye of common goldfish and was absent in ocular fluids or serum of mammalian species. This compound was isolated by chromatography on a carbohydrate column eluted by 10 mM ammonium phosphate followed by chromatography on a Delta PAK C18 column eluted by acetonitrile-water-trifluoroacetic acid. The ion size of the unknown compound determined by electrospray for protonated and deprotonated ions are m/z 205 and 203, respectively. The protonated ion was fragmented to m/z 187, 169, 157 and 141 as the cone voltage increased. The data from mass spectrometry and nuclear magnetic resonance spectroscopy indicate the chemical structure of a carbohydrate with 7 carbons containing a lactone ring.
Source Title: Spectroscopy
URI: https://scholarbank.nus.edu.sg/handle/10635/181127
ISSN: 07124813
DOI: 10.1155/2003/479764
Rights: Attribution 4.0 International
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