Please use this identifier to cite or link to this item: https://doi.org/10.1242/jcs.010322
Title: Ninein is released from the centrosome and moves bi-directionally along microtubules
Authors: Moss, D.K
Bellett, G
Carter, J.M
Liovic, M
Keynton, J
Prescott, A.R
Lane, E.B 
Mogensen, M.M
Keywords: cell protein
gamma tubulin
green fluorescent protein
kinesin
latrunculin B
ninein
nocodazole
unclassified drug
animal cell
article
cell adhesion
cell differentiation
cell junction
cellular distribution
centrosome
confocal microscopy
cytoplasm
epithelium cell
human
human cell
intracellular transport
microtubule
nonhuman
priority journal
protein function
protein localization
protein secretion
protein transport
Animals
Bicyclo Compounds, Heterocyclic
Cell Differentiation
Cell Line
Centrosome
Cytoplasm
Cytoskeletal Proteins
Ear, Inner
Epithelial Cells
Fluorescence Recovery After Photobleaching
Glycogen Synthase Kinase 3
Humans
Mice
Microtubules
Nocodazole
Nuclear Proteins
Recombinant Fusion Proteins
Thiazolidines
Tubulin Modulators
Issue Date: 2007
Citation: Moss, D.K, Bellett, G, Carter, J.M, Liovic, M, Keynton, J, Prescott, A.R, Lane, E.B, Mogensen, M.M (2007). Ninein is released from the centrosome and moves bi-directionally along microtubules. Journal of Cell Science 120 (17) : 3064-3074. ScholarBank@NUS Repository. https://doi.org/10.1242/jcs.010322
Rights: Attribution 4.0 International
Abstract: Cell-to-cell contact and polarisation of epithelial cells involve a major reorganisation of the microtubules and centrosomal components. The radial microtubule organisation is lost and an apico-basal array develops that is no longer anchored at the centrosome. This involves not only the relocation of microtubules but also of centrosomal anchoring proteins to apical non-centrosomal sites. The relocation of microtubule minus-end-anchoring proteins such as ninein to the apical sites is likely to be essential for the assembly and stabilisation of the apico-basal arrays in polarised epithelial cells. In this study, we establish that ninein is highly dynamic and that, in epithelial cells, it is present not only at the centrosome but also in the cytoplasm as distinct speckles. Live-cell imaging reveals that GFF-ninein speckles are released from the centrosome and move in a microtubule-dependent manner within the cytoplasm and thus establishes that epithelial cells possess the mechanical means for relocation of ninein to non-centrosomal anchoring sites. We also provide evidence for the deployment of ninein speckles to apical anchoring sites during epithelial differentiation in both an in situ tissue and an in vitro culture system. In addition, the findings suggest that the non-centrosomal microtubule anchoring sites associate with adherens junctions in polaxised epithelial cells.
Source Title: Journal of Cell Science
URI: https://scholarbank.nus.edu.sg/handle/10635/181033
ISSN: 0021-9533
DOI: 10.1242/jcs.010322
Rights: Attribution 4.0 International
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