Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13041-014-0092-8
Title: Protein tyrosine phosphatase receptor type R is required for Purkinje cell responsiveness in cerebellar long-term depression
Authors: Erkens, M
Tanaka-Yamamoto, K
Cheron, G
Márquez-Ruiz, J
Prigogine, C
Schepens, J.T
Nadif Kasri, N
Augustine, G.J 
Hendriks, W.J
Keywords: AMPA receptor
glutamate receptor ionotropic, AMPA 2
mitogen activated protein kinase
mitogen activated protein kinase kinase
protein tyrosine kinase
Ptprr protein, mouse
receptor like protein tyrosine phosphatase
animal
biological model
C57BL mouse
cerebellum
deficiency
electrostimulation
feedback system
female
knockout mouse
long term depression
male
metabolism
mouse mutant
phosphorylation
Purkinje cell
synapse
vibrissa
Animals
Cerebellum
Electric Stimulation
Extracellular Signal-Regulated MAP Kinases
Feedback, Physiological
Female
Long-Term Synaptic Depression
Male
Mice, Inbred C57BL
Mice, Knockout
Mice, Neurologic Mutants
Mitogen-Activated Protein Kinase Kinases
Models, Biological
Phosphorylation
Purkinje Cells
Receptor-Like Protein Tyrosine Phosphatases, Class 7
Receptors, AMPA
src-Family Kinases
Synapses
Vibrissae
Issue Date: 2015
Citation: Erkens, M, Tanaka-Yamamoto, K, Cheron, G, Márquez-Ruiz, J, Prigogine, C, Schepens, J.T, Nadif Kasri, N, Augustine, G.J, Hendriks, W.J (2015). Protein tyrosine phosphatase receptor type R is required for Purkinje cell responsiveness in cerebellar long-term depression. Molecular brain 8 : 1. ScholarBank@NUS Repository. https://doi.org/10.1186/s13041-014-0092-8
Rights: Attribution 4.0 International
Abstract: BACKGROUND: Regulation of synaptic connectivity, including long-term depression (LTD), allows proper tuning of cellular signalling processes within brain circuitry. In the cerebellum, a key centre for motor coordination, a positive feedback loop that includes mitogen-activated protein kinases (MAPKs) is required for proper temporal control of LTD at cerebellar Purkinje cell synapses. Here we report that the tyrosine-specific MAPK-phosphatase PTPRR plays a role in coordinating the activity of this regulatory loop.RESULTS: LTD in the cerebellum of Ptprr (-/-) mice is strongly impeded, in vitro and in vivo. Comparison of basal phospho-MAPK levels between wild-type and PTPRR deficient cerebellar slices revealed increased levels in mutants. This high basal phospho-MAPK level attenuated further increases in phospho-MAPK during chemical induction of LTD, essentially disrupting the positive feedback loop and preventing α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) phosphorylation and endocytosis.CONCLUSIONS: Our findings indicate an important role for PTPRR in maintaining low basal MAPK activity in Purkinje cells. This creates an optimal 'window' to boost MAPK activity following signals that induce LTD, which can then propagate through feed-forward signals to cause AMPAR internalization and LTD.
Source Title: Molecular brain
URI: https://scholarbank.nus.edu.sg/handle/10635/180920
ISSN: 17566606
DOI: 10.1186/s13041-014-0092-8
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s13041-014-0092-8.pdf1.43 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

8
checked on Sep 22, 2022

Page view(s)

146
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons