Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep17178
Title: Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis
Authors: Hua, F
Zhang, P
Zhang, F
Zhao, Y
Li, C
Sun, C
Wang, X
Yang, R
Wang, C
Yu, A
Zhou, L 
Keywords: animal
Bagg albino mouse
bioassay
female
flow kinetics
Francisella tularensis
isolation and purification
osmolarity
pH
procedures
sensitivity and specificity
standard
viscosity
Animals
Biological Assay
Female
Francisella tularensis
Hydrogen-Ion Concentration
Mice, Inbred BALB C
Osmolar Concentration
Reference Standards
Rheology
Sensitivity and Specificity
Viscosity
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Hua, F, Zhang, P, Zhang, F, Zhao, Y, Li, C, Sun, C, Wang, X, Yang, R, Wang, C, Yu, A, Zhou, L (2015). Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis. Scientific Reports 5 : 17178. ScholarBank@NUS Repository. https://doi.org/10.1038/srep17178
Rights: Attribution 4.0 International
Abstract: Francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay was 104CFU · mL-1 (100 CFU/test). The linear quantitative range covered five orders of magnitude, and the coefficients of variation were less than 10%. Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains. Samples with pH 2-13, high ion strengths (? 2 mol · L 1 solution of KCl and NaCl), high viscosities (? 50 mg · mL 1 PEG20000 or ? 20% glycerol), and high concentrations of biomacromolecules (? 400 mg · mL 1 bovine serum albumin or ? 80 mg · mL 1 casein) showed little influence on the assay. For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip. Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/180410
ISSN: 2045-2322
DOI: 10.1038/srep17178
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_srep17178.pdf1.55 MBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons