Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep17178
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dc.titleDevelopment and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis
dc.contributor.authorHua, F
dc.contributor.authorZhang, P
dc.contributor.authorZhang, F
dc.contributor.authorZhao, Y
dc.contributor.authorLi, C
dc.contributor.authorSun, C
dc.contributor.authorWang, X
dc.contributor.authorYang, R
dc.contributor.authorWang, C
dc.contributor.authorYu, A
dc.contributor.authorZhou, L
dc.date.accessioned2020-10-26T08:51:39Z
dc.date.available2020-10-26T08:51:39Z
dc.date.issued2015
dc.identifier.citationHua, F, Zhang, P, Zhang, F, Zhao, Y, Li, C, Sun, C, Wang, X, Yang, R, Wang, C, Yu, A, Zhou, L (2015). Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis. Scientific Reports 5 : 17178. ScholarBank@NUS Repository. https://doi.org/10.1038/srep17178
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/180410
dc.description.abstractFrancisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay was 104CFU · mL-1 (100 CFU/test). The linear quantitative range covered five orders of magnitude, and the coefficients of variation were less than 10%. Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains. Samples with pH 2-13, high ion strengths (? 2 mol · L 1 solution of KCl and NaCl), high viscosities (? 50 mg · mL 1 PEG20000 or ? 20% glycerol), and high concentrations of biomacromolecules (? 400 mg · mL 1 bovine serum albumin or ? 80 mg · mL 1 casein) showed little influence on the assay. For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip. Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectbioassay
dc.subjectfemale
dc.subjectflow kinetics
dc.subjectFrancisella tularensis
dc.subjectisolation and purification
dc.subjectosmolarity
dc.subjectpH
dc.subjectprocedures
dc.subjectsensitivity and specificity
dc.subjectstandard
dc.subjectviscosity
dc.subjectAnimals
dc.subjectBiological Assay
dc.subjectFemale
dc.subjectFrancisella tularensis
dc.subjectHydrogen-Ion Concentration
dc.subjectMice, Inbred BALB C
dc.subjectOsmolar Concentration
dc.subjectReference Standards
dc.subjectRheology
dc.subjectSensitivity and Specificity
dc.subjectViscosity
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.description.doi10.1038/srep17178
dc.description.sourcetitleScientific Reports
dc.description.volume5
dc.description.page17178
dc.published.statepublished
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