Please use this identifier to cite or link to this item:
https://doi.org/10.7554/eLife.03915
Title: | Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state | Authors: | Katz, Y Li, F Lambert, N.J Sokol, E.S Tam, W.-L Cheng, A.W Airoldi, E.M Lengner, C.J Gupta, P.B Yu, Z Jaenisch, R Burge, C.B |
Keywords: | Jagged1 Musashi protein RNA binding protein unclassified drug calcium binding protein ligand membrane protein nerve protein Notch receptor protein binding RNA binding protein Serrate proteins signal peptide Article cancer cell cell differentiation controlled study down regulation epithelial mesenchymal transition gene mutation gene overexpression genetic variability human human cell human tissue protein depletion protein expression RNA processing RNA splicing upregulation alternative RNA splicing animal biological model breast breast tumor epithelial mesenchymal transition epithelium cell female gene expression regulation genetic transcription genetics growth, development and aging knockout mouse metabolism molecular genetics morphogenesis neural stem cell nucleotide motif nucleotide sequence pathology protein synthesis tumor cell line Mammalia Alternative Splicing Animals Base Sequence Breast Breast Neoplasms Calcium-Binding Proteins Cell Line, Tumor Down-Regulation Epithelial Cells Epithelial-Mesenchymal Transition Female Gene Expression Regulation, Neoplastic Humans Intercellular Signaling Peptides and Proteins Ligands Membrane Proteins Mice, Knockout Models, Biological Molecular Sequence Data Morphogenesis Nerve Tissue Proteins Neural Stem Cells Nucleotide Motifs Protein Binding Protein Biosynthesis Receptors, Notch RNA-Binding Proteins Transcription, Genetic |
Issue Date: | 2014 | Citation: | Katz, Y, Li, F, Lambert, N.J, Sokol, E.S, Tam, W.-L, Cheng, A.W, Airoldi, E.M, Lengner, C.J, Gupta, P.B, Yu, Z, Jaenisch, R, Burge, C.B (2014). Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state. eLife 3 (November) : 1-27. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.03915 | Rights: | Attribution 4.0 International | Abstract: | The conserved Musashi (Msi) family of RNA binding proteins are expressed in stem/progenitor and cancer cells, but generally absent from differentiated cells, consistent with a role in cell state regulation. We found that Msi genes are rarely mutated but frequently overexpressed in human cancers and are associated with an epithelial-luminal cell state. Using ribosome profiling and RNA-seq analysis, we found that Msi proteins regulate translation of genes implicated in epithelial cell biology and epithelial-to-mesenchymal transition (EMT), and promote an epithelial splicing pattern. Overexpression of Msi proteins inhibited the translation of Jagged1, a factor required for EMT, and repressed EMT in cell culture and in mammary gland in vivo. Knockdown of Msis in epithelial cancer cells promoted loss of epithelial identity. Our results show that mammalian Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. © Katz et al. | Source Title: | eLife | URI: | https://scholarbank.nus.edu.sg/handle/10635/180375 | ISSN: | 2050084X | DOI: | 10.7554/eLife.03915 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_7554_eLife_03915.pdf | 4.29 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License