Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.03915
Title: Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state
Authors: Katz, Y
Li, F
Lambert, N.J
Sokol, E.S
Tam, W.-L 
Cheng, A.W
Airoldi, E.M
Lengner, C.J
Gupta, P.B
Yu, Z
Jaenisch, R
Burge, C.B
Keywords: Jagged1
Musashi protein
RNA binding protein
unclassified drug
calcium binding protein
ligand
membrane protein
nerve protein
Notch receptor
protein binding
RNA binding protein
Serrate proteins
signal peptide
Article
cancer cell
cell differentiation
controlled study
down regulation
epithelial mesenchymal transition
gene mutation
gene overexpression
genetic variability
human
human cell
human tissue
protein depletion
protein expression
RNA processing
RNA splicing
upregulation
alternative RNA splicing
animal
biological model
breast
breast tumor
epithelial mesenchymal transition
epithelium cell
female
gene expression regulation
genetic transcription
genetics
growth, development and aging
knockout mouse
metabolism
molecular genetics
morphogenesis
neural stem cell
nucleotide motif
nucleotide sequence
pathology
protein synthesis
tumor cell line
Mammalia
Alternative Splicing
Animals
Base Sequence
Breast
Breast Neoplasms
Calcium-Binding Proteins
Cell Line, Tumor
Down-Regulation
Epithelial Cells
Epithelial-Mesenchymal Transition
Female
Gene Expression Regulation, Neoplastic
Humans
Intercellular Signaling Peptides and Proteins
Ligands
Membrane Proteins
Mice, Knockout
Models, Biological
Molecular Sequence Data
Morphogenesis
Nerve Tissue Proteins
Neural Stem Cells
Nucleotide Motifs
Protein Binding
Protein Biosynthesis
Receptors, Notch
RNA-Binding Proteins
Transcription, Genetic
Issue Date: 2014
Citation: Katz, Y, Li, F, Lambert, N.J, Sokol, E.S, Tam, W.-L, Cheng, A.W, Airoldi, E.M, Lengner, C.J, Gupta, P.B, Yu, Z, Jaenisch, R, Burge, C.B (2014). Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state. eLife 3 (November) : Jan-27. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.03915
Rights: Attribution 4.0 International
Abstract: The conserved Musashi (Msi) family of RNA binding proteins are expressed in stem/progenitor and cancer cells, but generally absent from differentiated cells, consistent with a role in cell state regulation. We found that Msi genes are rarely mutated but frequently overexpressed in human cancers and are associated with an epithelial-luminal cell state. Using ribosome profiling and RNA-seq analysis, we found that Msi proteins regulate translation of genes implicated in epithelial cell biology and epithelial-to-mesenchymal transition (EMT), and promote an epithelial splicing pattern. Overexpression of Msi proteins inhibited the translation of Jagged1, a factor required for EMT, and repressed EMT in cell culture and in mammary gland in vivo. Knockdown of Msis in epithelial cancer cells promoted loss of epithelial identity. Our results show that mammalian Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. © Katz et al.
Source Title: eLife
URI: https://scholarbank.nus.edu.sg/handle/10635/180375
ISSN: 2050084X
DOI: 10.7554/eLife.03915
Rights: Attribution 4.0 International
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