Please use this identifier to cite or link to this item:
https://doi.org/10.7554/eLife.03915
DC Field | Value | |
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dc.title | Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state | |
dc.contributor.author | Katz, Y | |
dc.contributor.author | Li, F | |
dc.contributor.author | Lambert, N.J | |
dc.contributor.author | Sokol, E.S | |
dc.contributor.author | Tam, W.-L | |
dc.contributor.author | Cheng, A.W | |
dc.contributor.author | Airoldi, E.M | |
dc.contributor.author | Lengner, C.J | |
dc.contributor.author | Gupta, P.B | |
dc.contributor.author | Yu, Z | |
dc.contributor.author | Jaenisch, R | |
dc.contributor.author | Burge, C.B | |
dc.date.accessioned | 2020-10-26T08:33:45Z | |
dc.date.available | 2020-10-26T08:33:45Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Katz, Y, Li, F, Lambert, N.J, Sokol, E.S, Tam, W.-L, Cheng, A.W, Airoldi, E.M, Lengner, C.J, Gupta, P.B, Yu, Z, Jaenisch, R, Burge, C.B (2014). Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state. eLife 3 (November) : 1-27. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.03915 | |
dc.identifier.issn | 2050084X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/180375 | |
dc.description.abstract | The conserved Musashi (Msi) family of RNA binding proteins are expressed in stem/progenitor and cancer cells, but generally absent from differentiated cells, consistent with a role in cell state regulation. We found that Msi genes are rarely mutated but frequently overexpressed in human cancers and are associated with an epithelial-luminal cell state. Using ribosome profiling and RNA-seq analysis, we found that Msi proteins regulate translation of genes implicated in epithelial cell biology and epithelial-to-mesenchymal transition (EMT), and promote an epithelial splicing pattern. Overexpression of Msi proteins inhibited the translation of Jagged1, a factor required for EMT, and repressed EMT in cell culture and in mammary gland in vivo. Knockdown of Msis in epithelial cancer cells promoted loss of epithelial identity. Our results show that mammalian Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. © Katz et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | Jagged1 | |
dc.subject | Musashi protein | |
dc.subject | RNA binding protein | |
dc.subject | unclassified drug | |
dc.subject | calcium binding protein | |
dc.subject | ligand | |
dc.subject | membrane protein | |
dc.subject | nerve protein | |
dc.subject | Notch receptor | |
dc.subject | protein binding | |
dc.subject | RNA binding protein | |
dc.subject | Serrate proteins | |
dc.subject | signal peptide | |
dc.subject | Article | |
dc.subject | cancer cell | |
dc.subject | cell differentiation | |
dc.subject | controlled study | |
dc.subject | down regulation | |
dc.subject | epithelial mesenchymal transition | |
dc.subject | gene mutation | |
dc.subject | gene overexpression | |
dc.subject | genetic variability | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | human tissue | |
dc.subject | protein depletion | |
dc.subject | protein expression | |
dc.subject | RNA processing | |
dc.subject | RNA splicing | |
dc.subject | upregulation | |
dc.subject | alternative RNA splicing | |
dc.subject | animal | |
dc.subject | biological model | |
dc.subject | breast | |
dc.subject | breast tumor | |
dc.subject | epithelial mesenchymal transition | |
dc.subject | epithelium cell | |
dc.subject | female | |
dc.subject | gene expression regulation | |
dc.subject | genetic transcription | |
dc.subject | genetics | |
dc.subject | growth, development and aging | |
dc.subject | knockout mouse | |
dc.subject | metabolism | |
dc.subject | molecular genetics | |
dc.subject | morphogenesis | |
dc.subject | neural stem cell | |
dc.subject | nucleotide motif | |
dc.subject | nucleotide sequence | |
dc.subject | pathology | |
dc.subject | protein synthesis | |
dc.subject | tumor cell line | |
dc.subject | Mammalia | |
dc.subject | Alternative Splicing | |
dc.subject | Animals | |
dc.subject | Base Sequence | |
dc.subject | Breast | |
dc.subject | Breast Neoplasms | |
dc.subject | Calcium-Binding Proteins | |
dc.subject | Cell Line, Tumor | |
dc.subject | Down-Regulation | |
dc.subject | Epithelial Cells | |
dc.subject | Epithelial-Mesenchymal Transition | |
dc.subject | Female | |
dc.subject | Gene Expression Regulation, Neoplastic | |
dc.subject | Humans | |
dc.subject | Intercellular Signaling Peptides and Proteins | |
dc.subject | Ligands | |
dc.subject | Membrane Proteins | |
dc.subject | Mice, Knockout | |
dc.subject | Models, Biological | |
dc.subject | Molecular Sequence Data | |
dc.subject | Morphogenesis | |
dc.subject | Nerve Tissue Proteins | |
dc.subject | Neural Stem Cells | |
dc.subject | Nucleotide Motifs | |
dc.subject | Protein Binding | |
dc.subject | Protein Biosynthesis | |
dc.subject | Receptors, Notch | |
dc.subject | RNA-Binding Proteins | |
dc.subject | Transcription, Genetic | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.7554/eLife.03915 | |
dc.description.sourcetitle | eLife | |
dc.description.volume | 3 | |
dc.description.issue | November | |
dc.description.page | 1-27 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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