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https://doi.org/10.1155/2017/5864945
Title: | Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis | Authors: | Zhang, C.-L Lin, Z.-Q Luo, R.-J Zhang, X.-X Guo, J Wu, W Shi, N Deng, L.-H Chen, W.-W Zhang, X.-Y Bharucha, S Huang, W Sutton, R Windsor, J.A Xue, P Xia, Q |
Keywords: | adenylate cyclase arginine calcium ion carbachol chai qin cheng qi decoction fatty acid binding protein 2 messenger RNA motilin phospholipase C beta1 plant medicinal product protein kinase C protein kinase C alpha substance P unclassified drug vasoactive intestinal polypeptide acute hemorrhagic pancreatitis adult agar gel electrophoresis animal experiment animal model animal tissue Article calcium cell level calcium transport cell death cell infiltration cell isolation colonic muscle comparative study controlled study disease severity down regulation fluorescence histopathology inflammatory cell intestinal dysmotility intestine motility male nonhuman protein expression rat smooth muscle cell upregulation Western blotting |
Issue Date: | 2017 | Citation: | Zhang, C.-L, Lin, Z.-Q, Luo, R.-J, Zhang, X.-X, Guo, J, Wu, W, Shi, N, Deng, L.-H, Chen, W.-W, Zhang, X.-Y, Bharucha, S, Huang, W, Sutton, R, Windsor, J.A, Xue, P, Xia, Q (2017). Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis. Evidence-based Complementary and Alternative Medicine 2017 : 5864945. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/5864945 | Rights: | Attribution 4.0 International | Abstract: | Chai-Qin-Cheng-Qi decoction (CQCQD) improves intestinal motility in acute pancreatitis (AP), but the mechanism(s) require elucidation. We investigated the effects of CQCQD and carbachol, a prokinetic agent, on colonic smooth muscle cells (SMCs) in L-arginine-induced necrotising AP model in rats. In treatment groups, intragastric CQCQD (20 g/kg, 2 hourly × 3 doses) or intraperitoneal carbachol (60 μg/kg) was given 24 hours after induction of AP. Both CQCQD and carbachol decreased the severity of pancreatic and colonic histopathology (all P<0.05). Both CQCQD and carbachol reduced serum intestinal fatty acid binding protein, vasoactive intestinal peptide, and substance P and increased motility levels. CQCQD upregulated SMC phospholipase C-beta 1 (PLC-β1) mRNA and PLC protein (both P<0.05), while both treatments upregulated protein kinase C-alpha (PKC-α) mRNA and PKC protein and downregulated adenylate cyclase (AC) mRNA and protein compared with no treatment (all P<0.05). Neither treatment significantly altered L-arginine-induced PKC-β1 and PKC-ϵ mRNA reduction. Both treatments significantly increased fluorescence intensity of SMC intracellular calcium concentration [Ca2+]i (3563.5 and 3046.9 versus 1086.9, both P<0.01). These data suggest CQCQD and carbachol improve intestinal motility in AP by increasing [Ca2+]i in colonic SMCs via upregulating PLC, PKC and downregulating AC. © 2017 Chen-Long Zhang et al. | Source Title: | Evidence-based Complementary and Alternative Medicine | URI: | https://scholarbank.nus.edu.sg/handle/10635/179884 | ISSN: | 1741427X | DOI: | 10.1155/2017/5864945 | Rights: | Attribution 4.0 International |
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