Please use this identifier to cite or link to this item:
https://doi.org/10.1155/2017/5864945
DC Field | Value | |
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dc.title | Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis | |
dc.contributor.author | Zhang, C.-L | |
dc.contributor.author | Lin, Z.-Q | |
dc.contributor.author | Luo, R.-J | |
dc.contributor.author | Zhang, X.-X | |
dc.contributor.author | Guo, J | |
dc.contributor.author | Wu, W | |
dc.contributor.author | Shi, N | |
dc.contributor.author | Deng, L.-H | |
dc.contributor.author | Chen, W.-W | |
dc.contributor.author | Zhang, X.-Y | |
dc.contributor.author | Bharucha, S | |
dc.contributor.author | Huang, W | |
dc.contributor.author | Sutton, R | |
dc.contributor.author | Windsor, J.A | |
dc.contributor.author | Xue, P | |
dc.contributor.author | Xia, Q | |
dc.date.accessioned | 2020-10-26T04:57:56Z | |
dc.date.available | 2020-10-26T04:57:56Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Zhang, C.-L, Lin, Z.-Q, Luo, R.-J, Zhang, X.-X, Guo, J, Wu, W, Shi, N, Deng, L.-H, Chen, W.-W, Zhang, X.-Y, Bharucha, S, Huang, W, Sutton, R, Windsor, J.A, Xue, P, Xia, Q (2017). Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis. Evidence-based Complementary and Alternative Medicine 2017 : 5864945. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/5864945 | |
dc.identifier.issn | 1741427X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/179884 | |
dc.description.abstract | Chai-Qin-Cheng-Qi decoction (CQCQD) improves intestinal motility in acute pancreatitis (AP), but the mechanism(s) require elucidation. We investigated the effects of CQCQD and carbachol, a prokinetic agent, on colonic smooth muscle cells (SMCs) in L-arginine-induced necrotising AP model in rats. In treatment groups, intragastric CQCQD (20 g/kg, 2 hourly × 3 doses) or intraperitoneal carbachol (60 μg/kg) was given 24 hours after induction of AP. Both CQCQD and carbachol decreased the severity of pancreatic and colonic histopathology (all P<0.05). Both CQCQD and carbachol reduced serum intestinal fatty acid binding protein, vasoactive intestinal peptide, and substance P and increased motility levels. CQCQD upregulated SMC phospholipase C-beta 1 (PLC-β1) mRNA and PLC protein (both P<0.05), while both treatments upregulated protein kinase C-alpha (PKC-α) mRNA and PKC protein and downregulated adenylate cyclase (AC) mRNA and protein compared with no treatment (all P<0.05). Neither treatment significantly altered L-arginine-induced PKC-β1 and PKC-ϵ mRNA reduction. Both treatments significantly increased fluorescence intensity of SMC intracellular calcium concentration [Ca2+]i (3563.5 and 3046.9 versus 1086.9, both P<0.01). These data suggest CQCQD and carbachol improve intestinal motility in AP by increasing [Ca2+]i in colonic SMCs via upregulating PLC, PKC and downregulating AC. © 2017 Chen-Long Zhang et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | adenylate cyclase | |
dc.subject | arginine | |
dc.subject | calcium ion | |
dc.subject | carbachol | |
dc.subject | chai qin cheng qi decoction | |
dc.subject | fatty acid binding protein 2 | |
dc.subject | messenger RNA | |
dc.subject | motilin | |
dc.subject | phospholipase C beta1 | |
dc.subject | plant medicinal product | |
dc.subject | protein kinase C | |
dc.subject | protein kinase C alpha | |
dc.subject | substance P | |
dc.subject | unclassified drug | |
dc.subject | vasoactive intestinal polypeptide | |
dc.subject | acute hemorrhagic pancreatitis | |
dc.subject | adult | |
dc.subject | agar gel electrophoresis | |
dc.subject | animal experiment | |
dc.subject | animal model | |
dc.subject | animal tissue | |
dc.subject | Article | |
dc.subject | calcium cell level | |
dc.subject | calcium transport | |
dc.subject | cell death | |
dc.subject | cell infiltration | |
dc.subject | cell isolation | |
dc.subject | colonic muscle | |
dc.subject | comparative study | |
dc.subject | controlled study | |
dc.subject | disease severity | |
dc.subject | down regulation | |
dc.subject | fluorescence | |
dc.subject | histopathology | |
dc.subject | inflammatory cell | |
dc.subject | intestinal dysmotility | |
dc.subject | intestine motility | |
dc.subject | male | |
dc.subject | nonhuman | |
dc.subject | protein expression | |
dc.subject | rat | |
dc.subject | smooth muscle cell | |
dc.subject | upregulation | |
dc.subject | Western blotting | |
dc.type | Article | |
dc.contributor.department | SURGERY | |
dc.description.doi | 10.1155/2017/5864945 | |
dc.description.sourcetitle | Evidence-based Complementary and Alternative Medicine | |
dc.description.volume | 2017 | |
dc.description.page | 5864945 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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