Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep30397
Title: PP2ACdc55's role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function
Authors: Kerr, G.W
Wong, J.H
Arumugam, P 
Keywords: CDC14 protein, S cerevisiae
CDC55 protein, S cerevisiae
cell cycle protein
deoxyribonuclease
Net1 protein, S cerevisiae
nuclear protein
phosphoprotein phosphatase 2
protein tyrosine phosphatase
Saccharomyces cerevisiae protein
Spo11 protein, S cerevisiae
SPO12 protein, S cerevisiae
chromosome segregation
cytology
genetics
meiosis
metabolism
Saccharomyces cerevisiae
Cell Cycle Proteins
Chromosome Segregation
Endodeoxyribonucleases
Meiosis
Nuclear Proteins
Protein Phosphatase 2
Protein Tyrosine Phosphatases
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Issue Date: 2016
Citation: Kerr, G.W, Wong, J.H, Arumugam, P (2016). PP2ACdc55's role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function. Scientific Reports 6 : 30397. ScholarBank@NUS Repository. https://doi.org/10.1038/srep30397
Rights: Attribution 4.0 International
Abstract: PP2ACdc55 is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2ACdc55 activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2ACdc55 prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk. CDC55 was identified in a genetic screen for monopolins performed by isolating suppressors of spo11? spo12? lethality suggesting that Cdc55 might have a role in meiotic chromosome segregation. We investigated this possibility by isolating cdc55 alleles that suppress spo11? spo12? lethality and show that this suppression is independent of PP2ACdc55's FEAR function. Although the suppressor mutations in cdc55 affect reductional chromosome segregation in the absence of recombination, they have no effect on chromosome segregation during wild type meiosis. We suggest that Cdc55 is required for reductional chromosome segregation during achiasmate meiosis and this is independent of its FEAR function.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178886
ISSN: 20452322
DOI: 10.1038/srep30397
Rights: Attribution 4.0 International
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