Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep36993
Title: MRNA changes in nucleus accumbens related to methamphetamine addiction in mice
Authors: Zhu, L
Li, J
Dong, N
Guan, F
Liu, Y
Ma, D 
Goh, E.L.K 
Chen, T
Keywords: central stimulant agent
dopamine
long untranslated RNA
messenger RNA
methamphetamine
microRNA
transcriptome
alternative RNA splicing
amphetamine dependence
animal
C57BL mouse
comparative study
drug administration
drug effect
gene expression regulation
gene ontology
genetics
metabolism
motor activity
mouse
nerve cell plasticity
nucleus accumbens
randomization
reward
RNA processing
sequence alignment
sequence analysis
signal transduction
Alternative Splicing
Amphetamine-Related Disorders
Animals
Central Nervous System Stimulants
Dopamine
Drug Administration Schedule
Gene Expression Regulation
Gene Ontology
Methamphetamine
Mice
Mice, Inbred C57BL
MicroRNAs
Motor Activity
Neuronal Plasticity
Nucleus Accumbens
Random Allocation
Reward
RNA Processing, Post-Transcriptional
RNA, Long Noncoding
RNA, Messenger
Sequence Alignment
Sequence Analysis, RNA
Signal Transduction
Transcriptome
Issue Date: 2016
Citation: Zhu, L, Li, J, Dong, N, Guan, F, Liu, Y, Ma, D, Goh, E.L.K, Chen, T (2016). MRNA changes in nucleus accumbens related to methamphetamine addiction in mice. Scientific Reports 6 : 36993. ScholarBank@NUS Repository. https://doi.org/10.1038/srep36993
Rights: Attribution 4.0 International
Abstract: Methamphetamine (METH) is a highly addictive psychostimulant that elicits aberrant changes in the expression of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the nucleus accumbens of mice, indicating a potential role of METH in post-transcriptional regulations. To decipher the potential consequences of these post-transcriptional regulations in response to METH, we performed strand-specific RNA sequencing (ssRNA-Seq) to identify alterations in mRNA expression and their alternative splicing in the nucleus accumbens of mice following exposure to METH. METH-mediated changes in mRNAs were analyzed and correlated with previously reported changes in non-coding RNAs (miRNAs and lncRNAs) to determine the potential functions of these mRNA changes observed here and how non-coding RNAs are involved. A total of 2171 mRNAs were differentially expressed in response to METH with functions involved in synaptic plasticity, mitochondrial energy metabolism and immune response. 309 and 589 of these mRNAs are potential targets of miRNAs and lncRNAs respectively. In addition, METH treatment decreases mRNA alternative splicing, and there are 818 METH-specific events not observed in saline-treated mice. Our results suggest that METH-mediated addiction could be attributed by changes in miRNAs and lncRNAs and consequently, changes in mRNA alternative splicing and expression. In conclusion, our study reported a methamphetamine-modified nucleus accumbens transcriptome and provided non-coding RNA-mRNA interaction networks possibly involved in METH addiction. © The Author(s) 2016.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178756
ISSN: 20452322
DOI: 10.1038/srep36993
Rights: Attribution 4.0 International
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