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Title: MASTL is essential for anaphase entry of proliferating primordial germ cells and establishment of female germ cells in mice
Authors: Risal, S
Zhang, J
Adhikari, D
Liu, X
Shao, J
Hu, M
Busayavalasa, K
Tu, Z
Chen, Z
Kaldis, P 
Liu, K
Keywords: cyclin dependent kinase 1
histone H2AX
Ki 67 antigen
microtubule associated protein
microtubule associated serine threonine kinase like protein
protein serine threonine kinase
unclassified drug
animal cell
cell population
cell proliferation
cell structure
controlled study
embryo development
enzyme activity
female germline stem cell
mitosis inhibition
molecular dynamics
primordial germ cell
priority journal
promoter region
protein expression
protein phosphorylation
protein subunit
Issue Date: 2017
Citation: Risal, S, Zhang, J, Adhikari, D, Liu, X, Shao, J, Hu, M, Busayavalasa, K, Tu, Z, Chen, Z, Kaldis, P, Liu, K (2017). MASTL is essential for anaphase entry of proliferating primordial germ cells and establishment of female germ cells in mice. Cell Discovery 3 : 16052. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: In mammals, primordial germ cells (PGCs) are the embryonic cell population that serve as germ cell precursors in both females and males. During mouse embryonic development, the majority of PGCs are arrested at the G2 phase when they migrate into the hindgut at 7.75-8.75 dpc (days post coitum). It is after 9.5 dpc that the PGCs undergo proliferation with a doubling time of 12.6 h. The molecular mechanisms underlying PGC proliferation are however not well studied. In this work. Here we studied how MASTL (microtubule-associated serine/threonine kinase-like)/Greatwall kinase regulates the rapid proliferation of PGCs. We generated a mouse model where we specifically deleted Mastl in PGCs and found a significant loss of PGCs before the onset of meiosis in female PGCs. We further revealed that the deletion of Mastl in PGCs did not prevent mitotic entry, but led to a failure of the cells to proceed beyond metaphase-like stage, indicating that MASTL-mediated molecular events are indispensable for anaphase entry in PGCs. These mitotic defects further led to the death of Mastl-null PGCs by 12.5 dpc. Moreover, the defect in mitotic progression observed in the Mastl-null PGCs was rescued by simultaneous deletion of Ppp2r1a (? subunit of PP2A). Thus, our results demonstrate that MASTL, PP2A, and therefore regulated phosphatase activity have a fundamental role in establishing female germ cell population in gonads by controlling PGC proliferation during embryogenesis. © 2017 The Author(s).
Source Title: Cell Discovery
ISSN: 20565968
DOI: 10.1038/celldisc.2016.52
Rights: Attribution 4.0 International
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