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https://doi.org/10.1038/s41541-016-0003-3
Title: | A potent neutralizing antibody with therapeutic potential against all four serotypes of dengue virus | Authors: | Xu, M Zuest, R Velumani, S Tukijan, F Toh, Y.X Appanna, R Tan, E.Y Cerny, D MacAry, P Wang, C.-I Fink, K |
Issue Date: | 2017 | Citation: | Xu, M, Zuest, R, Velumani, S, Tukijan, F, Toh, Y.X, Appanna, R, Tan, E.Y, Cerny, D, MacAry, P, Wang, C.-I, Fink, K (2017). A potent neutralizing antibody with therapeutic potential against all four serotypes of dengue virus. npj Vaccines 2 (1) : 2. ScholarBank@NUS Repository. https://doi.org/10.1038/s41541-016-0003-3 | Rights: | Attribution 4.0 International | Abstract: | A therapy for dengue is still elusive. We describe the neutralizing and protective capacity of a dengue serotype-cross-reactive antibody isolated from the plasmablasts of a patient. Antibody SIgN-3C neutralized all four dengue virus serotypes at nano to picomolar concentrations and significantly decreased viremia of all serotypes in adult mice when given 2 days after infection. Moreover, mice were protected from pathology and death from a lethal dengue virus-2 infection. To avoid potential Fc-mediated uptake of immune complexes and ensuing enhanced infection, we introduced a LALA mutation in the Fc part. SIgN-3C-LALA was as efficient as the non-modified antibody in neutralizing dengue virus and in protecting mice while antibody-dependent enhancement was completely abrogated. The epitope of the antibody includes conserved amino acids in all three domains of the glycoprotein, which can explain its cross-reactivity. SIgN-3C-LALA neutralizes dengue virus both pre and post-attachment to host cells. These attributes likely contribute to the remarkable protective capacity of SIgN-3C. © 2017 The Author(s). | Source Title: | npj Vaccines | URI: | https://scholarbank.nus.edu.sg/handle/10635/178320 | ISSN: | 20590105 | DOI: | 10.1038/s41541-016-0003-3 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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