Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41541-016-0003-3
Title: A potent neutralizing antibody with therapeutic potential against all four serotypes of dengue virus
Authors: Xu, M
Zuest, R
Velumani, S
Tukijan, F
Toh, Y.X
Appanna, R
Tan, E.Y
Cerny, D
MacAry, P 
Wang, C.-I
Fink, K
Issue Date: 2017
Citation: Xu, M, Zuest, R, Velumani, S, Tukijan, F, Toh, Y.X, Appanna, R, Tan, E.Y, Cerny, D, MacAry, P, Wang, C.-I, Fink, K (2017). A potent neutralizing antibody with therapeutic potential against all four serotypes of dengue virus. npj Vaccines 2 (1) : 2. ScholarBank@NUS Repository. https://doi.org/10.1038/s41541-016-0003-3
Rights: Attribution 4.0 International
Abstract: A therapy for dengue is still elusive. We describe the neutralizing and protective capacity of a dengue serotype-cross-reactive antibody isolated from the plasmablasts of a patient. Antibody SIgN-3C neutralized all four dengue virus serotypes at nano to picomolar concentrations and significantly decreased viremia of all serotypes in adult mice when given 2 days after infection. Moreover, mice were protected from pathology and death from a lethal dengue virus-2 infection. To avoid potential Fc-mediated uptake of immune complexes and ensuing enhanced infection, we introduced a LALA mutation in the Fc part. SIgN-3C-LALA was as efficient as the non-modified antibody in neutralizing dengue virus and in protecting mice while antibody-dependent enhancement was completely abrogated. The epitope of the antibody includes conserved amino acids in all three domains of the glycoprotein, which can explain its cross-reactivity. SIgN-3C-LALA neutralizes dengue virus both pre and post-attachment to host cells. These attributes likely contribute to the remarkable protective capacity of SIgN-3C. © 2017 The Author(s).
Source Title: npj Vaccines
URI: https://scholarbank.nus.edu.sg/handle/10635/178320
ISSN: 20590105
DOI: 10.1038/s41541-016-0003-3
Rights: Attribution 4.0 International
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