Please use this identifier to cite or link to this item: https://doi.org/10.1186/scrt17
Title: Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: A longitudinal imaging study
Authors: Jackson, J.S
Golding, J.P
Chapon, C
Jones, W.A
Bhakoo, K.K 
Keywords: fluorescein isothiocyanate
glial fibrillary acidic protein
iron oxide
isolectin B4
nanoparticle
transcription factor Sox10
animal cell
animal experiment
animal model
article
autopsy
bone marrow cell
brain injury
cell differentiation
cell homing
cell lineage
cell migration
cell transplantation
cellular distribution
controlled study
corpus callosum
corpus striatum
encephalitis
fluorescence microscopy
glia cell
hair follicle
histopathology
immunohistochemistry
in situ hybridization
in vivo study
longitudinal study
male
minimally invasive procedure
neural crest cell
nonhuman
nuclear magnetic resonance imaging
phenotype
priority journal
rat
right hemisphere
stem cell
Administration, Intravenous
Animals
Bone Marrow Cells
Brain
Brain Injuries
Cell Differentiation
Cell Movement
Cell- and Tissue-Based Therapy
Corpus Callosum
Demyelinating Diseases
Ferric Compounds
Hair Follicle
Lipopolysaccharides
Magnetic Resonance Imaging
Metal Nanoparticles
Microscopy, Fluorescence
Neural Crest
Neurodegenerative Diseases
Neuroglia
Rats
Rats, Sprague-Dawley
SOXE Transcription Factors
Stem Cell Transplantation
Stem Cells
Transplantation, Autologous
Animalia
Rattus
Issue Date: 2010
Publisher: BMC
Citation: Jackson, J.S, Golding, J.P, Chapon, C, Jones, W.A, Bhakoo, K.K (2010). Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: A longitudinal imaging study. Stem Cell Research and Therapy 1 (2) : 17. ScholarBank@NUS Repository. https://doi.org/10.1186/scrt17
Rights: Attribution 4.0 International
Abstract: Introduction. This study aimed to determine the homing potential and fate of epidermal neural crest stem cells (eNCSCs) derived from hair follicles, and bone marrow-derived stem cells (BMSCs) of mesenchymal origin, in a lipopolysaccharide (LPS)-induced inflammatory lesion model in the rat brain. Both eNCSCs and BMSCs are easily accessible from adult tissues by using minimally invasive procedures and can differentiate into a variety of neuroglial lineages. Thus, these cells have the potential to be used in autologous cell-replacement therapies, minimizing immune rejection, and engineered to secrete a variety of molecules. Methods. Both eNCSCs and BMSCs were prelabeled with iron-oxide nanoparticles (IO-TAT-FITC) and implanted either onto the corpus callosum in healthy or LPS-lesioned animals or intravenously into lesioned animals. Both cell types were tracked longitudinally in vivo by using magnetic resonance imaging (MRI) for up to 30 days and confirmed by postmortem immunohistochemistry. Results: Transplanted cells in nonlesioned animals remained localized along the corpus callosum. Cells implanted distally from an LPS lesion (either intracerebrally or intravenously) migrated only toward the lesion, as seen by the localized MRI signal void. Fluorescence microscopy of the FITC tag on the nanoparticles confirmed the in vivo MRI data,. Conclusions: This study demonstrated that both cell types can be tracked in vivo by using noninvasive MRI and have pathotropic properties toward an inflammatory lesion in the brain. As these cells differentiate into the glial phenotype and are derived from adult tissues, they offer a viable alternative autologous stem cell source and gene-targeting potential for neurodegenerative and demyelinating pathologies. © 2010 Jackson et al.; licensee BioMed Central Ltd.
Source Title: Stem Cell Research and Therapy
URI: https://scholarbank.nus.edu.sg/handle/10635/178193
ISSN: 1757-6512
DOI: 10.1186/scrt17
Rights: Attribution 4.0 International
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