Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12985-018-0964-0
Title: Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species
Authors: Laing, E.D
Amaya, M
Navaratnarajah, C.K
Feng, Y.-R
Cattaneo, R
Wang, L.-F 
Broder, C.C
Keywords: ephrin B2
ephrin B3
green fluorescent protein
interferon
oligonucleotide
ephrin B2
green fluorescent protein
interferon
protein binding
virus envelope protein
virus receptor
animal cell
Article
controlled study
Henipavirus
human
human cell
in vitro study
interferon signaling pathway
luciferase assay
membrane fusion
microscopy
molecular cloning
nonhuman
recombinant Cedar virus
reverse genetics
signal transduction
split luciferase fusion assay
viral tropism
virus entry
virus recombinant
virus replication
wild type
cell fusion
cell line
cytopathogenic effect
genetic recombination
genetics
Henipavirus
Henipavirus infection
metabolism
pathogenicity
physiology
reporter gene
serodiagnosis
virology
Cell Fusion
Cell Line
Cytopathogenic Effect, Viral
Ephrin-B2
Genes, Reporter
Green Fluorescent Proteins
Henipavirus
Henipavirus Infections
Interferon Type I
Neutralization Tests
Protein Binding
Receptors, Virus
Recombination, Genetic
Reverse Genetics
Viral Envelope Proteins
Viral Tropism
Virus Internalization
Virus Replication
Issue Date: 2018
Citation: Laing, E.D, Amaya, M, Navaratnarajah, C.K, Feng, Y.-R, Cattaneo, R, Wang, L.-F, Broder, C.C (2018). Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species. Virology Journal 15 (1) : 56. ScholarBank@NUS Repository. https://doi.org/10.1186/s12985-018-0964-0
Rights: Attribution 4.0 International
Abstract: Background: Hendra virus and Nipah virus are zoonotic viruses that have caused severe to fatal disease in livestock and human populations. The isolation of Cedar virus, a non-pathogenic virus species in the genus Henipavirus, closely-related to the highly pathogenic Hendra virus and Nipah virus offers an opportunity to investigate differences in pathogenesis and receptor tropism among these viruses. Methods: We constructed full-length cDNA clones of Cedar virus from synthetic oligonucleotides and rescued two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that expresses a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. Replication kinetics of both viruses and stimulation of the interferon pathway were characterized in vitro. Cellular tropism for ephrin-B type ligands was qualitatively investigated by microscopy and quantitatively by a split-luciferase fusion assay. Results: Successful rescue of recombinant Cedar virus expressing a green fluorescent protein did not significantly affect virus replication compared to the recombinant wild-type Cedar virus. We demonstrated that recombinant Cedar virus stimulated the interferon pathway and utilized the established Hendra virus and Nipah virus receptor, ephrin-B2, but not ephrin-B3 to mediate virus entry. We further characterized virus-mediated membrane fusion kinetics of Cedar virus with the known henipavirus receptors ephrin-B2 and ephrin-B3. Conclusions: The recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals. Moreover, these experiments can be conducted safely under BSL-2 conditions. © 2018 The Author(s).
Source Title: Virology Journal
URI: https://scholarbank.nus.edu.sg/handle/10635/178096
ISSN: 1743422X
DOI: 10.1186/s12985-018-0964-0
Rights: Attribution 4.0 International
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