Please use this identifier to cite or link to this item:
https://doi.org/10.1186/s12985-018-0964-0
DC Field | Value | |
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dc.title | Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species | |
dc.contributor.author | Laing, E.D | |
dc.contributor.author | Amaya, M | |
dc.contributor.author | Navaratnarajah, C.K | |
dc.contributor.author | Feng, Y.-R | |
dc.contributor.author | Cattaneo, R | |
dc.contributor.author | Wang, L.-F | |
dc.contributor.author | Broder, C.C | |
dc.date.accessioned | 2020-10-20T05:05:09Z | |
dc.date.available | 2020-10-20T05:05:09Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Laing, E.D, Amaya, M, Navaratnarajah, C.K, Feng, Y.-R, Cattaneo, R, Wang, L.-F, Broder, C.C (2018). Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species. Virology Journal 15 (1) : 56. ScholarBank@NUS Repository. https://doi.org/10.1186/s12985-018-0964-0 | |
dc.identifier.issn | 1743422X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178096 | |
dc.description.abstract | Background: Hendra virus and Nipah virus are zoonotic viruses that have caused severe to fatal disease in livestock and human populations. The isolation of Cedar virus, a non-pathogenic virus species in the genus Henipavirus, closely-related to the highly pathogenic Hendra virus and Nipah virus offers an opportunity to investigate differences in pathogenesis and receptor tropism among these viruses. Methods: We constructed full-length cDNA clones of Cedar virus from synthetic oligonucleotides and rescued two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that expresses a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. Replication kinetics of both viruses and stimulation of the interferon pathway were characterized in vitro. Cellular tropism for ephrin-B type ligands was qualitatively investigated by microscopy and quantitatively by a split-luciferase fusion assay. Results: Successful rescue of recombinant Cedar virus expressing a green fluorescent protein did not significantly affect virus replication compared to the recombinant wild-type Cedar virus. We demonstrated that recombinant Cedar virus stimulated the interferon pathway and utilized the established Hendra virus and Nipah virus receptor, ephrin-B2, but not ephrin-B3 to mediate virus entry. We further characterized virus-mediated membrane fusion kinetics of Cedar virus with the known henipavirus receptors ephrin-B2 and ephrin-B3. Conclusions: The recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals. Moreover, these experiments can be conducted safely under BSL-2 conditions. © 2018 The Author(s). | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | ephrin B2 | |
dc.subject | ephrin B3 | |
dc.subject | green fluorescent protein | |
dc.subject | interferon | |
dc.subject | oligonucleotide | |
dc.subject | ephrin B2 | |
dc.subject | green fluorescent protein | |
dc.subject | interferon | |
dc.subject | protein binding | |
dc.subject | virus envelope protein | |
dc.subject | virus receptor | |
dc.subject | animal cell | |
dc.subject | Article | |
dc.subject | controlled study | |
dc.subject | Henipavirus | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | in vitro study | |
dc.subject | interferon signaling pathway | |
dc.subject | luciferase assay | |
dc.subject | membrane fusion | |
dc.subject | microscopy | |
dc.subject | molecular cloning | |
dc.subject | nonhuman | |
dc.subject | recombinant Cedar virus | |
dc.subject | reverse genetics | |
dc.subject | signal transduction | |
dc.subject | split luciferase fusion assay | |
dc.subject | viral tropism | |
dc.subject | virus entry | |
dc.subject | virus recombinant | |
dc.subject | virus replication | |
dc.subject | wild type | |
dc.subject | cell fusion | |
dc.subject | cell line | |
dc.subject | cytopathogenic effect | |
dc.subject | genetic recombination | |
dc.subject | genetics | |
dc.subject | Henipavirus | |
dc.subject | Henipavirus infection | |
dc.subject | metabolism | |
dc.subject | pathogenicity | |
dc.subject | physiology | |
dc.subject | reporter gene | |
dc.subject | serodiagnosis | |
dc.subject | virology | |
dc.subject | Cell Fusion | |
dc.subject | Cell Line | |
dc.subject | Cytopathogenic Effect, Viral | |
dc.subject | Ephrin-B2 | |
dc.subject | Genes, Reporter | |
dc.subject | Green Fluorescent Proteins | |
dc.subject | Henipavirus | |
dc.subject | Henipavirus Infections | |
dc.subject | Interferon Type I | |
dc.subject | Neutralization Tests | |
dc.subject | Protein Binding | |
dc.subject | Receptors, Virus | |
dc.subject | Recombination, Genetic | |
dc.subject | Reverse Genetics | |
dc.subject | Viral Envelope Proteins | |
dc.subject | Viral Tropism | |
dc.subject | Virus Internalization | |
dc.subject | Virus Replication | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1186/s12985-018-0964-0 | |
dc.description.sourcetitle | Virology Journal | |
dc.description.volume | 15 | |
dc.description.issue | 1 | |
dc.description.page | 56 | |
Appears in Collections: | Staff Publications Elements |
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