Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13287-019-1133-0
Title: Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway
Authors: Ning, T
Guo, J
Zhang, K
Li, K
Zhang, J
Yang, Z 
Ge, Z
Keywords: cyclic AMP responsive element binding protein
glycosaminoglycan
STAT3 protein
stress activated protein kinase
animal cell
animal experiment
animal model
animal tissue
Article
biological activity
bone marrow stroma cell
cartilage
cell culture technique
cell differentiation
cell stimulation
cell viability
chondrogenesis
chondropathy
comparative effectiveness
controlled study
electrostimulation
gene expression
gene expression level
histology
in vitro study
in vivo study
mesenchymal stem cell
nanotechnology
nonhuman
pig
priority journal
protein expression
pulsed electric field
rat
signal transduction
stem cell research
stem cell transplantation
tissue regeneration
upregulation
Issue Date: 2019
Citation: Ning, T, Guo, J, Zhang, K, Li, K, Zhang, J, Yang, Z, Ge, Z (2019). Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway. Stem Cell Research and Therapy 10 (1) : 45. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-019-1133-0
Rights: Attribution 4.0 International
Abstract: Background: Nanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is known about their effects on the differentiation of stem cells. Furthermore, mechanisms of electric fields on chondrogenic differentiation of mesenchymal stem cells (MSCs) remain elusive, and effects of electric fields on cartilage regeneration need to be verified in vivo. Here, we aimed to study the effects of nsPEFs on chondrogenic differentiation of MSCs in vitro and in vivo and further to explore the mechanisms behind the phenomenon. Methods: The effects of nsPEF-preconditioning on chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro were evaluated using cell viability, gene expression, glycosaminoglycan (sGAG) content, and histological staining, as well as in vivo cartilage regeneration in osteochondral defects of rats. Signaling pathways were investigated with protein expression and gene expression, respectively. Results: nsPEF-preconditioning with proper parameters (10 ns at 20 kV/cm, 100 ns at 10 kV/cm) significantly potentiated chondrogenic differentiation capacity of MSCs with upregulated cartilaginous gene expression and increased matrix deposition through activation of C-Jun NH2-terminal kinase (JNK) and cAMP-response element binding protein (CREB), followed by activation of downstream signal transducer and activator of transcription (STAT3). Implantation of nsPEF-preconditioned MSCs significantly enhanced cartilage regeneration in vivo, compared with implantation of non-nsPEF-preconditioned MSCs. Conclusion: This study demonstrates a unique approach of nsPEF treatment to potentiate the chondrogenic ability of MSCs through activation of JNK/CREB-STAT3 that could have translational potential for MSC-based cartilage regeneration. © 2019 The Author(s).
Source Title: Stem Cell Research and Therapy
URI: https://scholarbank.nus.edu.sg/handle/10635/178048
ISSN: 17576512
DOI: 10.1186/s13287-019-1133-0
Rights: Attribution 4.0 International
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