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https://doi.org/10.1186/s13287-019-1133-0
Title: | Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway | Authors: | Ning, T Guo, J Zhang, K Li, K Zhang, J Yang, Z Ge, Z |
Keywords: | cyclic AMP responsive element binding protein glycosaminoglycan STAT3 protein stress activated protein kinase animal cell animal experiment animal model animal tissue Article biological activity bone marrow stroma cell cartilage cell culture technique cell differentiation cell stimulation cell viability chondrogenesis chondropathy comparative effectiveness controlled study electrostimulation gene expression gene expression level histology in vitro study in vivo study mesenchymal stem cell nanotechnology nonhuman pig priority journal protein expression pulsed electric field rat signal transduction stem cell research stem cell transplantation tissue regeneration upregulation |
Issue Date: | 2019 | Citation: | Ning, T, Guo, J, Zhang, K, Li, K, Zhang, J, Yang, Z, Ge, Z (2019). Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway. Stem Cell Research and Therapy 10 (1) : 45. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-019-1133-0 | Rights: | Attribution 4.0 International | Abstract: | Background: Nanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is known about their effects on the differentiation of stem cells. Furthermore, mechanisms of electric fields on chondrogenic differentiation of mesenchymal stem cells (MSCs) remain elusive, and effects of electric fields on cartilage regeneration need to be verified in vivo. Here, we aimed to study the effects of nsPEFs on chondrogenic differentiation of MSCs in vitro and in vivo and further to explore the mechanisms behind the phenomenon. Methods: The effects of nsPEF-preconditioning on chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro were evaluated using cell viability, gene expression, glycosaminoglycan (sGAG) content, and histological staining, as well as in vivo cartilage regeneration in osteochondral defects of rats. Signaling pathways were investigated with protein expression and gene expression, respectively. Results: nsPEF-preconditioning with proper parameters (10 ns at 20 kV/cm, 100 ns at 10 kV/cm) significantly potentiated chondrogenic differentiation capacity of MSCs with upregulated cartilaginous gene expression and increased matrix deposition through activation of C-Jun NH2-terminal kinase (JNK) and cAMP-response element binding protein (CREB), followed by activation of downstream signal transducer and activator of transcription (STAT3). Implantation of nsPEF-preconditioned MSCs significantly enhanced cartilage regeneration in vivo, compared with implantation of non-nsPEF-preconditioned MSCs. Conclusion: This study demonstrates a unique approach of nsPEF treatment to potentiate the chondrogenic ability of MSCs through activation of JNK/CREB-STAT3 that could have translational potential for MSC-based cartilage regeneration. © 2019 The Author(s). | Source Title: | Stem Cell Research and Therapy | URI: | https://scholarbank.nus.edu.sg/handle/10635/178048 | ISSN: | 17576512 | DOI: | 10.1186/s13287-019-1133-0 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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