Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13287-019-1133-0
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dc.titleNanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway
dc.contributor.authorNing, T
dc.contributor.authorGuo, J
dc.contributor.authorZhang, K
dc.contributor.authorLi, K
dc.contributor.authorZhang, J
dc.contributor.authorYang, Z
dc.contributor.authorGe, Z
dc.date.accessioned2020-10-20T04:56:04Z
dc.date.available2020-10-20T04:56:04Z
dc.date.issued2019
dc.identifier.citationNing, T, Guo, J, Zhang, K, Li, K, Zhang, J, Yang, Z, Ge, Z (2019). Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway. Stem Cell Research and Therapy 10 (1) : 45. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-019-1133-0
dc.identifier.issn17576512
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178048
dc.description.abstractBackground: Nanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is known about their effects on the differentiation of stem cells. Furthermore, mechanisms of electric fields on chondrogenic differentiation of mesenchymal stem cells (MSCs) remain elusive, and effects of electric fields on cartilage regeneration need to be verified in vivo. Here, we aimed to study the effects of nsPEFs on chondrogenic differentiation of MSCs in vitro and in vivo and further to explore the mechanisms behind the phenomenon. Methods: The effects of nsPEF-preconditioning on chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro were evaluated using cell viability, gene expression, glycosaminoglycan (sGAG) content, and histological staining, as well as in vivo cartilage regeneration in osteochondral defects of rats. Signaling pathways were investigated with protein expression and gene expression, respectively. Results: nsPEF-preconditioning with proper parameters (10 ns at 20 kV/cm, 100 ns at 10 kV/cm) significantly potentiated chondrogenic differentiation capacity of MSCs with upregulated cartilaginous gene expression and increased matrix deposition through activation of C-Jun NH2-terminal kinase (JNK) and cAMP-response element binding protein (CREB), followed by activation of downstream signal transducer and activator of transcription (STAT3). Implantation of nsPEF-preconditioned MSCs significantly enhanced cartilage regeneration in vivo, compared with implantation of non-nsPEF-preconditioned MSCs. Conclusion: This study demonstrates a unique approach of nsPEF treatment to potentiate the chondrogenic ability of MSCs through activation of JNK/CREB-STAT3 that could have translational potential for MSC-based cartilage regeneration. © 2019 The Author(s).
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectcyclic AMP responsive element binding protein
dc.subjectglycosaminoglycan
dc.subjectSTAT3 protein
dc.subjectstress activated protein kinase
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbiological activity
dc.subjectbone marrow stroma cell
dc.subjectcartilage
dc.subjectcell culture technique
dc.subjectcell differentiation
dc.subjectcell stimulation
dc.subjectcell viability
dc.subjectchondrogenesis
dc.subjectchondropathy
dc.subjectcomparative effectiveness
dc.subjectcontrolled study
dc.subjectelectrostimulation
dc.subjectgene expression
dc.subjectgene expression level
dc.subjecthistology
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectmesenchymal stem cell
dc.subjectnanotechnology
dc.subjectnonhuman
dc.subjectpig
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectpulsed electric field
dc.subjectrat
dc.subjectsignal transduction
dc.subjectstem cell research
dc.subjectstem cell transplantation
dc.subjecttissue regeneration
dc.subjectupregulation
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.description.doi10.1186/s13287-019-1133-0
dc.description.sourcetitleStem Cell Research and Therapy
dc.description.volume10
dc.description.issue1
dc.description.page45
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