Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-018-24269-3
Title: Cryopreservation of Fish Spermatogonial Cells: The Future of Natural History Collections
Authors: Hagedorn, M.M
Daly, J.P
Carter, V.L
Cole, K.S
Jaafar, Z 
Lager, C.V.A
Parenti, L.R
Keywords: animal
cryopreservation
cytology
fish
male
procedures
specimen handling
spermatogonium
testis
Animals
Cryopreservation
Fishes
Male
Specimen Handling
Spermatogonia
Testis
Issue Date: 2018
Citation: Hagedorn, M.M, Daly, J.P, Carter, V.L, Cole, K.S, Jaafar, Z, Lager, C.V.A, Parenti, L.R (2018). Cryopreservation of Fish Spermatogonial Cells: The Future of Natural History Collections. Scientific Reports 8 (1) : 6149. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-24269-3
Rights: Attribution 4.0 International
Abstract: As global biodiversity declines, the value of biological collections increases. Cryopreserved diploid spermatogonial cells meet two goals: to yield high-quality molecular sequence data; and to regenerate new individuals, hence potentially countering species extinction. Cryopreserved spermatogonial cells that allow for such mitigative measures are not currently in natural history museum collections because there are no standard protocols to collect them. Vertebrate specimens, especially fishes, are traditionally formalin-fixed and alcohol-preserved which makes them ideal for morphological studies and as museum vouchers, but inadequate for molecular sequence data. Molecular studies of fishes routinely use tissues preserved in ethanol; yet tissues preserved in this way may yield degraded sequences over time. As an alternative to tissue fixation methods, we assessed and compared previously published cryopreservation methods by gating and counting fish testicular cells with flow cytometry to identify presumptive spermatogonia A-type cells. Here we describe a protocol to cryopreserve tissues that yields a high percentage of viable spermatogonial cells from the testes of Asterropteryx semipunctata, a marine goby. Material cryopreserved using this protocol represents the first frozen and post-thaw viable spermatogonial cells of fishes archived in a natural history museum to provide better quality material for re-derivation of species and DNA preservation and analysis. © 2018 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/177820
ISSN: 20452322
DOI: 10.1038/s41598-018-24269-3
Rights: Attribution 4.0 International
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