Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep04477
Title: Super-resolution microscopy reveals decondensed chromatin structure at transcription sites
Authors: Wang, Y 
Maharana, S
Wang, M.D
Shivashankar, G.V 
Keywords: DNA
histone
RNA polymerase II
article
chemistry
chromatin
chromatin assembly and disassembly
genetic transcription
genetics
HeLa cell line
human
microscopy
transcription initiation
ultrastructure
Chromatin
Chromatin Assembly and Disassembly
DNA
HeLa Cells
Histones
Humans
Microscopy
RNA Polymerase II
Transcription, Genetic
Transcriptional Activation
Issue Date: 2014
Publisher: Nature Publishing Groups
Citation: Wang, Y, Maharana, S, Wang, M.D, Shivashankar, G.V (2014). Super-resolution microscopy reveals decondensed chromatin structure at transcription sites. Scientific Reports 4 : 4477. ScholarBank@NUS Repository. https://doi.org/10.1038/srep04477
Rights: Attribution 4.0 International
Abstract: Remodeling of the local chromatin structure is essential for the regulation of gene expression. While a number of biochemical and bioimaging experiments suggest decondensed chromatin structures are associated with transcription, a direct visualization of DNA and transcriptionally active RNA polymerase II (RNA pol II) at super-resolution is still lacking. Here we investigate the structure of chromatin isolated from HeLa cells using binding activatable localization microscopy (BALM). The sample preparation method preserved the structural integrity of chromatin. Interestingly, BALM imaging of the chromatin spreads revealed the presence of decondensed chromatin as gap structures along the spreads. These gaps were enriched with phosphorylated S5 RNA pol II, and were sensitive to the cellular transcriptional state. Taken together, we could visualize the decondensed chromatin regions together with active RNA pol II for the first time using super-resolution microscopy.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/177768
ISSN: 20452322
DOI: 10.1038/srep04477
Rights: Attribution 4.0 International
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